Dengue, Yellow Fever, Zika and Chikungunya epidemic arboviruses in Brazil: ultrastructural aspects

The impact of arbovirus cocirculation in Brazil is unknown. Dengue virus (DENV) reinfection may result in more intense viraemia or immunopathology, leading to more severe disease. The Zika virus (ZIKV) epidemic in the Americas provided pathogenicity evidence that had not been previously observed in...

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Published in:Memórias do Instituto Oswaldo Cruz 2021, Vol.115, p.e200278-e200278
Main Authors: Barreto-Vieira, Debora Ferreira, Couto-Lima, Dinair, Jácome, Fernanda Cunha, Caldas, Gabriela Cardoso, Barth, Ortrud Monika
Format: Article
Language:English
Subjects:
Animals
Arboviruses
Brazil - epidemiology
Chikungunya Fever - epidemiology
Chlorocebus aethiops
Dengue - epidemiology
Epidemics
flaviviruses
Original
PARASITOLOGY
transmission electron microscopy
TROPICAL MEDICINE
Vero Cells
viroplasm
Yellow Fever
Zika Virus
Zika Virus Infection - epidemiology
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Summary:The impact of arbovirus cocirculation in Brazil is unknown. Dengue virus (DENV) reinfection may result in more intense viraemia or immunopathology, leading to more severe disease. The Zika virus (ZIKV) epidemic in the Americas provided pathogenicity evidence that had not been previously observed in flavivirus infections. In contrast to other flaviviruses, electron microscopy studies have shown that ZIKV may replicate in viroplasm-like structures. Flaviviruses produce an ensemble of structurally different virions, collectively contributing to tissue tropism and virus dissemination. In this work, the Aedes albopictus mosquito cell lineage (C6/36 cells) and kidney epithelial cells from African green monkeys (Vero cells) were infected with samples of the main circulating arboviruses in Brazil [DENV-1, DENV-2, DENV-3, DENV-4, ZIKV, Yellow Fever virus (YFV) and Chikungunya virus (CHIKV)], and ultrastructural studies by transmission electron microscopy were performed. We observed that ZIKV, the DENV serotypes, YFV and CHIKV particles are spherical. ZIKV, DENV-1, -2, -3 and -4 presented diameters of 40-50 nm, and CHIKV presented approximate diameters of 50-60 nm. Viroplasm-like structures was observed in ZIKV replication cycle. The morphogenesis of these arboviruses is similar to what has been presented in previous studies. However, we understand that further studies are needed to investigate the relationship between viroplasm-like structures and ZIKV replication dynamics.
ISSN:0074-0276
1678-8060
1678-8060
DOI:10.1590/0074-02760200278