Updated 16S rRNA-RFLP method for the identification of all currently characterised Arcobacter spp

Arcobacter spp. (family Campylobacteraceae) are ubiquitous zoonotic bacteria that are being increasingly recognised as a threat to human health. A previously published 16S rRNA-RFLP Arcobacter spp. identification method produced specific RFLP patterns for the six species described at that time, usin...

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Published in:BMC microbiology 2012-12, Vol.12 (1), p.292-292
Main Authors: Figueras, María José, Levican, Arturo, Collado, Luis
Format: Article
Language:English
Subjects:
16S rRNA gene mutations
16S rRNA-RFLP
Agarose
Arcobacter
Arcobacter - classification
Arcobacter - genetics
Arcobacter cryaerophilus
Bacteriological Techniques - methods
Computer simulation
Digestion
DNA Restriction Enzymes - metabolism
DNA, Bacterial - genetics
Electrophoresis, Agar Gel
Electrophoresis, Polyacrylamide Gel
Endonuclease
Feces
Gel electrophoresis
Genes
Identification
Methodology
Methods
Microbiology
Microheterogeneity
Molecular weight
Mutation
Polyacrylamide
Polymorphism, Restriction Fragment Length
RNA, Bacterial - genetics
RNA, Ribosomal, 16S - genetics
rRNA 16S
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Levican, Arturo
Collado, Luis
description Arcobacter spp. (family Campylobacteraceae) are ubiquitous zoonotic bacteria that are being increasingly recognised as a threat to human health. A previously published 16S rRNA-RFLP Arcobacter spp. identification method produced specific RFLP patterns for the six species described at that time, using a single endonuclease (MseI). The number of characterised Arcobacter species has since risen to 17. The aim of the present study was to update the 16S rRNA-RFLP identification method to include all currently characterised species of Arcobacter. Digestion of the 16S rRNA gene with the endonuclease MseI produced clear, distinctive patterns for 10 of the 17 species, while the remaining species shared a common or very similar RFLP pattern. Subsequent digestion of the 16S rRNA gene from these species with the endonucleases MnlI and/or BfaI generated species-specific RFLP patterns. 16S rRNA-RFLP analysis identified 17 Arcobacter spp. using either polyacrylamide or agarose gel electrophoresis. Microheterogeneities within the 16S rRNA gene, which interfered with the RFLP identification, were also documented for the first time in this genus, particularly in strains of Arcobacter cryaerophilus isolated from animal faeces and aborted foetuses.
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source Open Access: PubMed Central; Publicly Available Content Database
subjects 16S rRNA gene mutations
16S rRNA-RFLP
Agarose
Arcobacter
Arcobacter - classification
Arcobacter - genetics
Arcobacter cryaerophilus
Bacteriological Techniques - methods
Computer simulation
Digestion
DNA Restriction Enzymes - metabolism
DNA, Bacterial - genetics
Electrophoresis, Agar Gel
Electrophoresis, Polyacrylamide Gel
Endonuclease
Feces
Gel electrophoresis
Genes
Identification
Methodology
Methods
Microbiology
Microheterogeneity
Molecular weight
Mutation
Polyacrylamide
Polymorphism, Restriction Fragment Length
RNA, Bacterial - genetics
RNA, Ribosomal, 16S - genetics
rRNA 16S
title Updated 16S rRNA-RFLP method for the identification of all currently characterised Arcobacter spp
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