Adenovirus transduction of 3T3-L1 cells

3T3-L1 cells offer an excellent model system for studies of differentiation and biochemistry of fat cells. However, these cells are limited in their utility by the low efficiency with which DNA can be introduced by transfection. Gene delivery by viral vectors, particularly adenovirus, has proven a p...

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Bibliographic Details
Published in:Journal of lipid research 2001-03, Vol.42 (3), p.460-466
Main Authors: Orlicky, D J, Schaack, J
Format: Article
Language:English
Subjects:
3T3 Cells
adenoviral vectors
Adenoviridae - genetics
adipocyte lipid homeostasis
Adipocytes - metabolism
Animals
Cation Exchange Resins - pharmacology
Cell Differentiation
Cytomegalovirus - genetics
Fluorescence
Genetic Vectors
Green Fluorescent Proteins
Indicators and Reagents
Lipid Metabolism
Lipids - pharmacology
Luminescent Proteins - genetics
Mice
Polylysine - pharmacology
transduction efficiency
Transfection
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Summary:3T3-L1 cells offer an excellent model system for studies of differentiation and biochemistry of fat cells. However, these cells are limited in their utility by the low efficiency with which DNA can be introduced by transfection. Gene delivery by viral vectors, particularly adenovirus, has proven a powerful means for introduction of genes into certain cell types. Furthermore, adenovirus transduction has been used to study mechanisms involved in the differentiation of 3T3-L1 cells into mature fat cells. We show in this study that 3T3-L1 cells are inefficiently transduced by adenovirus. The potential advantages offered by adenovirus transduction led us to examine methods designed to enhance transduction of 3T3-L1 cells by adenovirus. Of these methods, polylysine-mediated enhancement demonstrates considerable promise because it permits up to 100% of cells to be transduced and because it does not inhibit differentiation of 3T3-L1 cells. -- Orlicky D. J., and J. Schaack. Adenovirus transduction of 3T3-L1 cells. J. Lipid Res. 2001. 42: 460--466.
ISSN:0022-2275
DOI:10.1016/S0022-2275(20)31671-0