A Novel Drastic Peptide Genetically Adapted to Biomimetic Scaffolds "Delivers" Osteogenic Signals to Human Mesenchymal Stem Cells

This work describes the design, preparation, and deep investigation of "intelligent nanobiomaterials" that fulfill the safety rules and aim to serve as "signal deliverers" for osteogenesis, harboring a specific peptide that promotes and enhances osteogenesis at the end of their h...

Full description

Saved in:
Bibliographic Details
Published in:Nanomaterials (Basel, Switzerland) Switzerland), 2023-03, Vol.13 (7), p.1236
Main Authors: Mantsou, Aglaia, Papachristou, Eleni, Keramidas, Panagiotis, Lamprou, Paraskevas, Pavlidis, Alexandros, Papi, Rigini M, Dimitriou, Katerina, Aggeli, Amalia, Choli-Papadopoulou, Theodora
Format: Article
Language:English
Subjects:
Analysis
biomaterial
Biomaterials
Biomedical materials
Biomimetics
BMP-2 peptide
Bone matrix
Bone morphogenetic protein 2
Bone morphogenetic protein receptor type II
bone regeneration
Bones
Cartilage
Cbfa-1 protein
Chemical synthesis
Collagen (type I)
E coli
Elastin
Extracellular matrix
Fibers
Glycoproteins
Growth
Hydrogels
Identification and classification
Mechanical properties
Mesenchymal stem cells
Methods
Mineralization
Nanomaterials
Nanoparticles
Osteocalcin
Osteogenesis
Peptides
Phase transitions
Phosphatase
Phosphorylation
Plasmids
Polypeptides
Properties
Proteins
scaffold
Scaffolds
Silk fibroin
Skin
Smad protein
Stem cells
tissue engineering
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This work describes the design, preparation, and deep investigation of "intelligent nanobiomaterials" that fulfill the safety rules and aim to serve as "signal deliverers" for osteogenesis, harboring a specific peptide that promotes and enhances osteogenesis at the end of their hydrogel fibers. The de novo synthesized protein fibers, besides their mechanical properties owed to their protein constituents from elastin, silk fibroin and mussel-foot adhesive protein-1 as well as to cell-attachment peptides from extracellular matrix glycoproteins, incorporate the Bone Morphogenetic Protein-2 (BMP2) peptide (AISMLYLDEN) that, according to our studies, serves as "signal deliverer" for osteogenesis. The osteogenetic capacity of the biomaterial has been evidenced by investigating the osteogenic marker genes , , , , , and , which were increased drastically in cells cultured on scaffold-BMP2 for 21 days, even in the absence of osteogenesis medium. In addition, the induction of phosphorylation of intracellular Smad-1/5 and Erk-1/2 proteins clearly supported the osteogenetic capacity of the biomaterial.
ISSN:2079-4991
2079-4991
DOI:10.3390/nano13071236