Cumulative expression of heterologous XlnR regulatory modules and AraRA731V in Penicillium oxalicum enhances saccharification efficiency of corn stover and corn fiber

• Published in: Biotechnology for biofuels 2024-02, Vol.17 (1), p.18-18, Article 18
• Main Authors: Xia, Chengqiang, Qi, Xiaoyu, Song, Xin
• Format: Article
• Language: English
• Subjects: alpha-N-arabinofuranosidase; Arabinofuranosidase; biofuels; Cellulase; Cellulose; Corn; Corn fibers; corn stover; Crop yields; Diseases and pests; Efficiency; Enzymes; Filamentous fungi; Fungi; Genes; Glucose; Hemicellulase; Lignocellulose; Management; Modules; Mutants; Penicillium oxalicum; Production management; Raw materials; Saccharification; Stover; Sugar; sugars; Transcription factor AraR; Vegetables; XlnR regulatory modules; Xylosidase
• ISSN: 2731-3654; 2731-3654; 1754-6834
• DOI: 10.1186/s13068-024-02464-x

Penicillium oxalicum engineered strain DB2 and its mutant strains with multiple regulatory modules were constructed. Mutant strain RE-4-2 with two regulatory modules showed a significant increase in the reducing sugar released from corn stover and corn fiber as well as in the conversion of cellulose than DB2. RE-5-2 with three regulatory modules showed a further increase in reducing sugar released from corn stover and the conversion of cellulose on the basis of RE-4-2. RE-4-2-AraRA731V constructed by overexpressing AraRA731V in RE-4-2 showed an increase of 7.2 times and 1.2 times in arabinofuranosidase and xylosidase activities, respectively. Reducing sugar yield and cellulose conversion of corn stover and corn fiber by RE-4-2-AraRA731V were further increased.