A Modified Miniscope System for Simultaneous Electrophysiology and Calcium Imaging in vivo

The miniscope system is one of the calcium (Ca 2+ ) imaging tools with small size and lightweight and can realize the deep-brain Ca 2+ imaging not confined to the cerebral cortex. Combining Ca 2+ imaging and electrophysiology recording has been an efficient method for extracting high temporal-spatia...

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Bibliographic Details
Published in:Frontiers in integrative neuroscience 2021-08, Vol.15, p.682019-682019
Main Authors: Wu, Xiaoting, Yang, Xiangyu, Song, Lulu, Wang, Yang, Li, Yamin, Liu, Yuanyuan, Yang, Xiaowei, Wang, Yijun, Pei, Weihua, Li, Weidong
Format: Article
Language:English
Subjects:
Aluminum
Brain research
Calcium imaging
Calcium signalling
Cerebral cortex
Chemical vapor deposition
Cortex (temporal)
Design
Electrodes
Electrophysiological recording
Electrophysiology
electrophysiology recording
Graphene
high resolution
Microelectromechanical systems
Microscopy
modified miniscope system
Neuroimaging
Neurons
Neuroscience
Neurosciences
probe
Silicon wafers
Spatial discrimination
Stress concentration
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Summary:The miniscope system is one of the calcium (Ca 2+ ) imaging tools with small size and lightweight and can realize the deep-brain Ca 2+ imaging not confined to the cerebral cortex. Combining Ca 2+ imaging and electrophysiology recording has been an efficient method for extracting high temporal-spatial resolution signals in the brain. In this study, a particular electrode probe was developed and assembled on the imaging lens to modify the miniscope system. The electrode probe can be tightly integrated into the lens of the miniscope without increasing the volume, weight, and implantation complexity. In vivo tests verified that the proposed modified system has realized the simultaneous recording of Ca 2+ signals and local field potential (LFP) signal in the hippocampus CA1 region of an adult mouse.
ISSN:1662-5145
1662-5145
DOI:10.3389/fnint.2021.682019