Monitoring SARS-CoV-2 Surrogate TGEV Individual Virions Structure Survival under Harsh Physicochemical Environments

Effective airborne transmission of coronaviruses via liquid microdroplets requires a virion structure that must withstand harsh environmental conditions. Due to the demanding biosafety requirements for the study of human respiratory viruses, it is important to develop surrogate models to facilitate...

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Bibliographic Details
Published in:Cells (Basel, Switzerland) Switzerland), 2022-05, Vol.11 (11), p.1759
Main Authors: Cantero, Miguel, Carlero, Diego, Chichón, Francisco Javier, Martín-Benito, Jaime, De Pablo, Pedro José
Format: Article
Language:English
Subjects:
Biocides
coronavirus
Coronaviruses
COVID-19
Detergents - pharmacology
disinfection
Environmental conditions
Gastroenteritis
Humans
Hygiene
Mechanical properties
Pandemics
physical virology
SARS-CoV-2
Severe acute respiratory syndrome coronavirus 2
Transmissible gastroenteritis
Transmissible gastroenteritis virus - metabolism
uncoating
Virion - metabolism
Virions
Viruses
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Summary:Effective airborne transmission of coronaviruses via liquid microdroplets requires a virion structure that must withstand harsh environmental conditions. Due to the demanding biosafety requirements for the study of human respiratory viruses, it is important to develop surrogate models to facilitate their investigation. Here we explore the mechanical properties and nanostructure of transmissible gastroenteritis virus (TGEV) virions in liquid milieu and their response to different chemical agents commonly used as biocides. Our data provide two-fold results on virus stability: First, while particles with larger size and lower packing fraction kept their morphology intact after successive mechanical aggressions, smaller viruses with higher packing fraction showed conspicuous evidence of structural damage and content release. Second, monitoring the structure of single TGEV particles in the presence of detergent and alcohol in real time revealed the stages of gradual degradation of the virus structure in situ. These data suggest that detergent is three orders of magnitude more efficient than alcohol in destabilizing TGEV virus particles, paving the way for optimizing hygienic protocols for viruses with similar structure, such as SARS-CoV-2.
ISSN:2073-4409
2073-4409
DOI:10.3390/cells11111759