Multi-omics analysis of soil microbiota and metabolites in dryland wheat fields under different tillage methods

• Published in: Scientific reports 2024-10, Vol.14 (1), p.24066-15, Article 24066
• Main Authors: Dong, Fei, Wang, Longxing, Xu, Tianjing, Yan, Qiuyan, Yan, Shuangdui, Li, Feng, Chen, Lihao, Zhang, Ruifu
• Format: Article
• Language: English
• Subjects: 16S rRNA gene sequencing; 631/326/2565/2134; 631/45/320; Agriculture - methods; Arid zones; Bacteria - classification; Bacteria - genetics; Bacteria - metabolism; Biosynthesis; Correlation analysis; Differential metabolites; Fungi - genetics; Fungi - metabolism; Humanities and Social Sciences; ITS sequencing; Metabolites; Metabolome; Metabolomics; Metabolomics - methods; Microbial activity; Microbiomes; Microbiota; Microorganisms; multidisciplinary; Multiomics; Rhizosphere; Rhizosphere microorganisms; Science; Science (multidisciplinary); Secondary metabolites; Soil - chemistry; Soil aggregates; Soil analysis; Soil fertility; Soil Microbiology; Soil microorganisms; Sucrose; Synergistic effect; Tillage; Triticum - growth & development; Triticum - metabolism; Wheat; Winter wheat
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-74620-0

No-tillage and subsoiling can improve soil aggregate structure and realize a synergistic effect of soil carbon and nitrogen retention compared with deep tillage. This study aimed to investigate the effects of different tillage methods on the microbiome and metabolites in wheat rhizosphere. Results indicated that no significant differences in the diversity of soil bacterial and fungal communities were observed among the tillage methods. Analysis revealed that no-tillage enriched specific genera such as Cryptosporangium , Crossiella , Rhodothermaceae , Leptothrix , Stilbella , Diutina , and Pyrenochaetopsis , while subsoiling was associated with Rubrobacter , Latescibacteraceae , Nitrospira , Rokubacteriales , and Ctenomyces . Deep tillage, on the other hand, showed significant associations with Nocardia , Aeromicrobium , Sphingopyxis , Cordyceps , and Subulicystidium . Metabolomic analysis identified differential metabolites involved in various pathways, including the biosynthesis of plant secondary metabolites, ABC transporters, and starch and sucrose metabolism. Correlation analysis revealed a significant interaction between microorganisms and metabolites in wheat rhizosphere. Bacteria at the genus level exhibited greater associations with differential metabolites. In conclusion, different tillage practices can alter the composition of microbial communities and metabolites in wheat rhizosphere, and their interactions may affect soil fertility and wheat growth.