CCR3 antagonist impairs estradiol-induced eosinophil migration to the uterus in ovariectomized mice

Eosinophils are abundant in the reproductive tract, contributing to the remodeling and successful implantation of the embryo. However, the mechanisms by which eosinophils migrate into the uterus and their relationship to edema are still not entirely clear, since there are a variety of chemotactic fa...

Full description

Saved in:
Bibliographic Details
Published in:Brazilian journal of medical and biological research 2020-01, Vol.53 (1), p.e8659-e8659
Main Authors: Araújo, J M D, Silva, L A S, Felix, F B, Camargo, E A, Grespan, R
Format: Article
Language:English
Subjects:
17β-Estradiol
Animals
BIOLOGY
Cell Movement - drug effects
Chemokine receptor
Chemotactic factors
Edema
Eosinophils - drug effects
Estradiol
Estradiol - administration & dosage
Estrogens - administration & dosage
Female
Hypertrophy
Immunology
Leukocyte migration
Leukocytes (eosinophilic)
MEDICINE, RESEARCH & EXPERIMENTAL
Menopause
Mice
Mice, Inbred C57BL
Ovariectomy
Peroxidase
Receptors, CCR3 - antagonists & inhibitors
Reproductive system
Uterus
Uterus - cytology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Eosinophils are abundant in the reproductive tract, contributing to the remodeling and successful implantation of the embryo. However, the mechanisms by which eosinophils migrate into the uterus and their relationship to edema are still not entirely clear, since there are a variety of chemotactic factors that can cause migration of these cells. Therefore, to evaluate the role of CCR3 in eosinophil migration, ovariectomized C57BL/6 mice were treated with CCR3 antagonist SB 328437 and 17β-estradiol. The hypothesis that the CCR3 receptor plays an important role in eosinophil migration to the mouse uterus was confirmed, because we observed reduction in eosinophil peroxidase activity in these antagonist-treated uteruses. The antagonist also influenced uterine hypertrophy, inhibiting edema formation. Finally, histological analysis of the orcein-stained uteruses showed that the antagonist reduced eosinophil migration together with edema. These data showed that the CCR3 receptor is an important target for studies that seek to clarify the functions of these cells in uterine physiology.
ISSN:0100-879X
1414-431X
1414-431X
1678-4510
DOI:10.1590/1414-431x20198659