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Use of Autochthonous Lactiplantibacillus plantarum Strains to Produce Fermented Fish Products
The present research was aimed to the optimization of the production of a fish fermented product using autochthonous starters. The activity was performed through two phases: (1) Optimization of fermented fish product composition by using a 2 Fractional Factorial Design: the variables tested were nit...
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Published in: | Frontiers in microbiology 2020-12, Vol.11, p.615904-615904 |
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Main Authors: | , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | The present research was aimed to the optimization of the production of a fish fermented
product using autochthonous
starters. The activity was performed through two phases: (1) Optimization of fermented fish product composition by using a 2
Fractional Factorial Design: the variables tested were nitrites (0-150 ppm), salt (2.5-7.5%), sucrose (0-4%), white pepper (0-0.10%), and fermentation temperature (10-30°C); (2) Product realization and evaluation of its microbiological profile [aerobic microbiota (APC),
(PSE),
(E), and lactic acid bacteria (LAB) populations], chemico-physical parameters (pH and a
), and sensorial quality (odor, texture, color, and overall acceptability) during its storage at 4°C for 21 days. In the first step, the fish pulp was mixed with the appropriate amounts of ingredients, according to the experimental design; each batch was individually inoculated with the studied starter (
11,
69, and
DSM1055) at 10
cfu/g and incubated at 10, 20, or 30°C for 7 days. The lowest fermentation time (time to reach pH 4.4) was obtained with 4% sucrose, 100 ppm nitrite and a process temperature of 30°C. In the second step,
were produced according to the individuated formulation and inoculated with the studied starters (10
cfu/g); the fish mixture was stuffed into a natural casing and left to ferment at 30°C for 7 days. The use of the selected strains not only assured a correct fermentation but reduced the process time at only 2 days; during refrigerated storage, a good microbiological, chemico-physical and sensorial quality of the final product was recorded for at least 21 days. |
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ISSN: | 1664-302X 1664-302X |
DOI: | 10.3389/fmicb.2020.615904 |