Effect of oral administration of microcin Y on growth performance, intestinal barrier function and gut microbiota of chicks challenged with Salmonella Pullorum

• Published in: Veterinary research (Paris) 2024-05, Vol.55 (1), p.66-66, Article 66
• Main Authors: Li, Wenjing, Zeng, Zhiwei, Zhou, Di, Wang, Guyao, Wang, Zepeng, Li, Yu, Han, Yu, Qin, Miaomiao, Luo, Changqi, Feng, Saixiang, Cao, Weisheng
• Format: Article
• Language: English
• Subjects: Administration, Oral; Animal Feed - analysis; Animals; Anti-Bacterial Agents - administration & dosage; Anti-Bacterial Agents - pharmacology; Antibacterial agents; antibacterial properties; average daily gain; Bacteriocins - administration & dosage; Bacteriocins - pharmacology; birds; blood serum; Body weight; cecum; Chickens; Contamination; Escherichia; feces; flora; Gastrointestinal Microbiome - drug effects; growth performance; gut microbiota; Health aspects; ileum; Infection; interleukin-10; interleukin-4; intestinal barrier; Intestinal Barrier Function; intestinal microorganisms; jejunum; Life Sciences; liver; Microbiota (Symbiotic organisms); Microcin Y; oral administration; poultry; Poultry Diseases - microbiology; probiotics; Random Allocation; Salmonella; Salmonella - drug effects; Salmonella - physiology; Salmonella Infections, Animal - microbiology; Salmonella Pullorum; serotypes; specific pathogen-free animals; Specific Pathogen-Free Organisms; spleen; villi
• ISSN: 1297-9716; 0928-4249; 1297-9716
• DOI: 10.1186/s13567-024-01321-x

The lasso peptide microcin Y (MccY) effectively inhibits various serotypes of Salmonella in vitro, but the antibacterial effect against S. Pullorum in poultry is still unclear. This study was the first to evaluate the safety and anti-S. Pullorum infection of MccY in specific pathogen-free (SPF) chicks. The safety test showed that the body weight, IgA and IgM levels of serum, and cecal microbiota structure of 3 groups of chicks orally administrated with different doses of MccY (5 mg/kg, 10 mg/kg, 20 mg/kg) for 14 days were not significantly different from those of the control group. Then, the chicks were randomized into 3 groups for the experiment of anti-S. Pullorum infection: (I) negative control group (NC), (II) S. Pullorum-challenged group (SP, 5 × 10  CFU/bird), (III) MccY-treated group (MccY, 20 mg/kg). The results indicated that compared to the SP group, treatment of MccY increased body weight and average daily gain (P