Knockdown of ephrin-A5 expression by 40% does not affect motor axon growth or migration into the chick hindlimb

Bidirectional signaling between Eph receptor tyrosine kinases and their cell-surface protein signals, the ephrins, comprises one mechanism for guiding motor axons to their proper targets. During projection of motor axons from the lateral motor column (LMC) motor neurons of the spinal cord to the hin...

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Bibliographic Details
Published in:International journal of molecular sciences 2011-12, Vol.12 (12), p.8362-8371
Main Authors: Winning, Robert S, Krull, Catherine E
Format: Article
Language:English
Subjects:
Animals
Axon guidance
Axons - metabolism
Axons - physiology
Cell migration
Cell Movement
chick
Chick Embryo
Eph protein
Ephrin-A5 - genetics
Ephrin-A5 - metabolism
ephrins
Hindlimb - innervation
Hypotheses
in ovo electroporation
Kinases
Limb buds
Motor neurons
Motor Neurons - cytology
Motor Neurons - metabolism
Motor Neurons - physiology
Muscle, Skeletal - innervation
Muscles
Nerves
Neurogenesis
Protein-tyrosine kinase
Proteins
Spinal cord
Spinal Cord - cytology
Spinal Cord - embryology
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Summary:Bidirectional signaling between Eph receptor tyrosine kinases and their cell-surface protein signals, the ephrins, comprises one mechanism for guiding motor axons to their proper targets. During projection of motor axons from the lateral motor column (LMC) motor neurons of the spinal cord to the hindlimb muscles in chick embryos, ephrin-A5 has been shown to be expressed in the LMC motor axons until they reach the base of the limb bud and initiate sorting into their presumptive dorsal and ventral nerve trunks, at which point expression is extinguished. We tested the hypothesis that this dynamic pattern of ephrin-A5 expression in LMC motor axons is important for the growth and guidance of the axons to, and into, the hindlimb by knocking down endogenous ephrin-A5 expression in the motor neurons and their axons. No perturbation of LMC motor axon projections was observed in response to this treatment, suggesting that ephrin-A5 is not needed for LMC motor axon growth or guidance.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms12128362