Random Lasing Detection of Mutant Huntingtin Expression in Cells

Huntington’s disease (HD) is an autosomal dominant, incurable neurodegenerative disease caused by mutation in the huntingtin gene (HTT). HTT mutation leads to protein misfolding and aggregation, which affect cells’ functions and structural features. Because these changes might modify the scattering...

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Bibliographic Details
Published in:Sensors (Basel, Switzerland) Switzerland), 2021-05, Vol.21 (11), p.3825
Main Authors: de Armas-Rillo, Sergio, Fumagallo-Reading, Felipe, Luis-Ravelo, Diego, Abdul-Jalbar, Beatriz, González-Hernández, Tomás, Lahoz, Fernando
Format: Article
Language:English
Subjects:
Biomarkers
Cell culture
Cloning
Discriminant analysis
Huntingtons disease
Huntington’s disease
Lasers
Lasing
LDA
Multivariate statistical analysis
Mutation
optical diagnostics
optical sensor
PCA
Principal components analysis
random lasing
Statistical analysis
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Summary:Huntington’s disease (HD) is an autosomal dominant, incurable neurodegenerative disease caused by mutation in the huntingtin gene (HTT). HTT mutation leads to protein misfolding and aggregation, which affect cells’ functions and structural features. Because these changes might modify the scattering strength of affected cells, we propose that random lasing (RL) is an appropriate technique for detecting cells that express mutated HTT. To explore this hypothesis, we used a cell model of HD based on the expression of two different forms—pathogenic and non-pathogenic—of HTT. The RL signals from both cell profiles were compared. A multivariate statistical analysis of the RL signals based on the principal component analysis (PCA) and linear discriminant analysis (LDA) techniques revealed substantial differences between cells that expressed the pathogenic and the non-pathogenic forms of HTT.
ISSN:1424-8220
1424-8220
DOI:10.3390/s21113825