Integrative analysis of gene expression, protein abundance, and metabolomic profiling elucidates complex relationships in chronic hyperglycemia-induced changes in human aortic smooth muscle cells

Type 2 diabetes mellitus (T2DM) is a major public health concern with significant cardiovascular complications (CVD). Despite extensive epidemiological data, the molecular mechanisms relating hyperglycemia to CVD remain incompletely understood. We here investigated the impact of chronic hyperglycemi...

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Bibliographic Details
Published in:Journal of biological engineering 2024-10, Vol.18 (1), p.61-19, Article 61
Main Authors: Bohara, Smriti, Bagheri, Atefeh, Ertugral, Elif G, Radzikh, Igor, Sandlers, Yana, Jiang, Peng, Kothapalli, Chandrasekhar R
Format: Article
Language:English
Subjects:
Atomic force microscopy
Biomechanics
Complications and side effects
Dextrose
Diabetes
Epidemiology
Gene expression
Genes
Glucose
Growth factors
Hyperglycemia
International economic relations
Muscle proteins
Prediabetic state
Scientific equipment and supplies industry
Smooth muscle
Transcriptomics
Type 2 diabetes
Vascular endothelial growth factor
Vascular smooth muscle cells
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Summary:Type 2 diabetes mellitus (T2DM) is a major public health concern with significant cardiovascular complications (CVD). Despite extensive epidemiological data, the molecular mechanisms relating hyperglycemia to CVD remain incompletely understood. We here investigated the impact of chronic hyperglycemia on human aortic smooth muscle cells (HASMCs) cultured under varying glucose conditions in vitro, mimicking normal (5 mmol/L), pre-diabetic (10 mmol/L), and diabetic (20 mmol/L) conditions, respectively. Normal HASMC cultures served as baseline controls, and patient-derived T2DM-SMCs served as disease controls. Results showed significant increases in cellular proliferation, area, perimeter, and F-actin expression with increasing glucose concentration (p 
ISSN:1754-1611
1754-1611
DOI:10.1186/s13036-024-00457-w