Melatonin Modifies Histone Acetylation During In Vitro Maturation of Mouse Oocytes

We evaluated the effect of melatonin, as a potent antioxidant agent, on glutathione (GSH) and reactive oxygen species (ROS) levels, as well as histone H3 lysine 9 (H3K9), and H4 lysine 12 (H4K12) acetylation when added to oocytes culture medium. In this experimental study, two in vitro and in vivo g...

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Published in:Cell journal (Yakhteh) 2018-07, Vol.20 (2), p.244-249
Main Authors: Keshavarzi, Somayeh, Salehi, Mohammad, Farifteh-Nobijari, Fattaneh, Hosseini, Taher, Hosseini, Sara, Ghazifard, Alaleh, Ghaffari Novin, Marefat, Fallah-Omrani, Vahid, Nourozian, Mohsen, Hosseini, Ahmad
Format: Article
Language:English
Subjects:
Acetylation
Aging
Antibodies
Antioxidants
Apoptosis
Cell cycle
Chromatin
Deoxyribonucleic acid
Dimethyl sulfoxide
DNA
DNA methylation
Embryos
Enzymes
Epigenetics
Free radicals
Gametocytes
Gene expression
Glutathione
Histone H3
Histones
In vitro fertilization
In Vitro Oocyte Maturation
In vivo methods and tests
Lysine
Maturation
Melatonin
Mice
Oocytes
Original
Ovaries
Oxidative stress
Reactive Oxygen Species
Staining
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Summary:We evaluated the effect of melatonin, as a potent antioxidant agent, on glutathione (GSH) and reactive oxygen species (ROS) levels, as well as histone H3 lysine 9 (H3K9), and H4 lysine 12 (H4K12) acetylation when added to oocytes culture medium. In this experimental study, two in vitro and in vivo groups were used. In the in vitro group, cumulus oocyte complexes (COCs) from the ovaries of B6D2F1 mice were cultured in maturation medium containing two doses of melatonin (10 and 10 M) and without melatonin [control group treated with dimethyl sulfoxide (DMSO)] for 22-24 hour. The cumulus expansion and nuclear status were monitored by an inverted microscope. Next, COCs were isolated from the oviducts of superovulated mice and studied as the in vivo group. In in vitro and in vivo matured oocytes, GSH and ROS levels were assessed by monochlorobimane (MCB) and 2-7-dichlorodihydrofluorescein diacetate (H2DCFDA) staining, respectively. Changes in histone acetylation were examined by immunofluorescent staining with specific antibodies against acetylated H3K9 and H4K12. The H4K12 acetylation and ROS levels were significantly higher in the oocytes matured in the in vitro group compared to the in vivo group (P
ISSN:2228-5806
2228-5814
DOI:10.22074/cellj.2018.4860