Circulating cell-free DNA (cfDNA) in patients with medullary thyroid carcinoma is characterized by specific fragmentation and methylation changes with diagnostic value

Medullary Thyroid Carcinoma (MTC) is a rare neuroendocrine tumour whose diagnosis includes evaluating calcitonin serum levels, which can present fluctuations unrelated to MTC. Here, we investigated circulating DNA fragmentation and methylation changes as potential biomarkers using ddPCR on cell-free...

Full description

Saved in:
Bibliographic Details
Published in:Biomarker research 2023-09, Vol.11 (1), p.1-82, Article 82
Main Authors: Citarella, Anna, Besharat, Zein Mersini, Trocchianesi, Sofia, Autilio, Tanja Milena, Verrienti, Antonella, Catanzaro, Giuseppina, Splendiani, Elena, Spinello, Zaira, Cantara, Silvia, Zavattari, Patrizia, Loi, Eleonora, Romei, Cristina, Ciampi, Raffaele, Pezzullo, Luciano, Castagna, Maria Grazia, Angeloni, Antonio, Elisei, Rosella, Durante, Cosimo, Po, Agnese, Ferretti, Elisabetta
Format: Article
Language:English
Subjects:
Analysis
Biomarkers
Calcitonin
Cancer
Carcinoma
Cell-free DNA
Circulating biomarker
Circulating DNA fragmentation
Circulating DNA methylation
Correspondence
Diagnosis
DNA
DNA fragmentation
DNA methylation
Epigenetics
Genetic aspects
Internet
Medullary thyroid carcinoma
Methylation
Mutation
Neuroendocrine tumors
Rare tumour
Remission
Serum levels
Size distribution
Surgery
Thyroid cancer
Thyroid carcinoma
Thyroid diseases
Trends
Tumors
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites cdi_FETCH-LOGICAL-c526t-7e39dd3d0add0316cc4337bef878719643c2437a55dd19628815fbe0054caf523
container_end_page 82
container_issue 1
container_start_page 1
container_title Biomarker research
container_volume 11
creator Citarella, Anna
Besharat, Zein Mersini
Trocchianesi, Sofia
Autilio, Tanja Milena
Verrienti, Antonella
Catanzaro, Giuseppina
Splendiani, Elena
Spinello, Zaira
Cantara, Silvia
Zavattari, Patrizia
Loi, Eleonora
Romei, Cristina
Ciampi, Raffaele
Pezzullo, Luciano
Castagna, Maria Grazia
Angeloni, Antonio
Elisei, Rosella
Durante, Cosimo
Po, Agnese
Ferretti, Elisabetta
description Medullary Thyroid Carcinoma (MTC) is a rare neuroendocrine tumour whose diagnosis includes evaluating calcitonin serum levels, which can present fluctuations unrelated to MTC. Here, we investigated circulating DNA fragmentation and methylation changes as potential biomarkers using ddPCR on cell-free DNA (cfDNA) isolated from the plasma of MTC patients. For cfDNA fragmentation analysis, we investigated the fragment size distribution of a gene family and calculated short fragment fraction (SFF). Methylation analyses evaluated the methylation levels of CG_16698623, a CG dinucleotide in the MGMT gene that we found hypermethylated in MTC tissues by analyzing public databases. The SFF ratio and methylation of CG_16698623 were significantly increased in plasma from MTC patients at diagnosis, and patients with clinical remission or stable disease at follow-up showed no significant SFF difference compared with healthy subjects. Our data support the diagnostic value of cfDNA traits that could enable better management of MTC patients.
doi_str_mv 10.1186/s40364-023-00522-4
format article
fullrecord <record><control><sourceid>gale_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_5d194c6b6274431c8479e3b83625ff68</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A765943394</galeid><doaj_id>oai_doaj_org_article_5d194c6b6274431c8479e3b83625ff68</doaj_id><sourcerecordid>A765943394</sourcerecordid><originalsourceid>FETCH-LOGICAL-c526t-7e39dd3d0add0316cc4337bef878719643c2437a55dd19628815fbe0054caf523</originalsourceid><addsrcrecordid>eNptkttu1DAQhiMEotXSF-DKEjftRYrP9l6hquVQqQKJw7Xl9SHrKmsvdlIoL8RrMu1WwCIcWRPb_3zO_Jmue07wKSFavmwcM8l7TFmPsaC054-6Q4oF7pVS5PFf7wfdUWvXGIbWnJDl0-6AKUWlpuqw-3meqptHO6U8IBfGsY81BHTx_gwduwjhBKWMtnAe8tTQtzSt0Sb4eRxtvUXT-raW5JGz1aVcNhalhtzaVuumUNOP4NHqFrVtcCkmh2K1wwY4QCsZ2ewBBYhxt4a8PISHO3yyQy5tgqwbO87hWfck2rGFo4e46L68ef35_F1_9eHt5fnZVe8ElVOvAlt6zzy23mNGpHOcMbUKUSutyFJy5ihnygrhPSyp1kTEVQAHubNRULboLndcX-y12da0gTpNscncb5Q6GFvhq8ZgBBC4kytJFeeMOM3VMrCVZpKKGKUG1qsdazuvwDMHlVc77kH3T3Jam6HcGIIFwVRJIBw_EGr5Ooc2mU1qd3_J5lDmZqiWUgnNmADpi3-k12WuGbwClVJ8KbAmf1SDhQpSjgUudndQc6akWIJbMBfd6X9U8PiwSa7kEBPs7yWc7CWAZgrfp8HOrZnLTx_3tXSndbW0VkP8bQjB5q61za61DbS2uW9tw9kvVCTp4g</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2877495081</pqid></control><display><type>article</type><title>Circulating cell-free DNA (cfDNA) in patients with medullary thyroid carcinoma is characterized by specific fragmentation and methylation changes with diagnostic value</title><source>Open Access: PubMed Central</source><source>Publicly Available Content Database (Proquest) (PQ_SDU_P3)</source><creator>Citarella, Anna ; Besharat, Zein Mersini ; Trocchianesi, Sofia ; Autilio, Tanja Milena ; Verrienti, Antonella ; Catanzaro, Giuseppina ; Splendiani, Elena ; Spinello, Zaira ; Cantara, Silvia ; Zavattari, Patrizia ; Loi, Eleonora ; Romei, Cristina ; Ciampi, Raffaele ; Pezzullo, Luciano ; Castagna, Maria Grazia ; Angeloni, Antonio ; Elisei, Rosella ; Durante, Cosimo ; Po, Agnese ; Ferretti, Elisabetta</creator><creatorcontrib>Citarella, Anna ; Besharat, Zein Mersini ; Trocchianesi, Sofia ; Autilio, Tanja Milena ; Verrienti, Antonella ; Catanzaro, Giuseppina ; Splendiani, Elena ; Spinello, Zaira ; Cantara, Silvia ; Zavattari, Patrizia ; Loi, Eleonora ; Romei, Cristina ; Ciampi, Raffaele ; Pezzullo, Luciano ; Castagna, Maria Grazia ; Angeloni, Antonio ; Elisei, Rosella ; Durante, Cosimo ; Po, Agnese ; Ferretti, Elisabetta</creatorcontrib><description>Medullary Thyroid Carcinoma (MTC) is a rare neuroendocrine tumour whose diagnosis includes evaluating calcitonin serum levels, which can present fluctuations unrelated to MTC. Here, we investigated circulating DNA fragmentation and methylation changes as potential biomarkers using ddPCR on cell-free DNA (cfDNA) isolated from the plasma of MTC patients. For cfDNA fragmentation analysis, we investigated the fragment size distribution of a gene family and calculated short fragment fraction (SFF). Methylation analyses evaluated the methylation levels of CG_16698623, a CG dinucleotide in the MGMT gene that we found hypermethylated in MTC tissues by analyzing public databases. The SFF ratio and methylation of CG_16698623 were significantly increased in plasma from MTC patients at diagnosis, and patients with clinical remission or stable disease at follow-up showed no significant SFF difference compared with healthy subjects. Our data support the diagnostic value of cfDNA traits that could enable better management of MTC patients.</description><identifier>ISSN: 2050-7771</identifier><identifier>EISSN: 2050-7771</identifier><identifier>DOI: 10.1186/s40364-023-00522-4</identifier><identifier>PMID: 37726827</identifier><language>eng</language><publisher>London: BioMed Central Ltd</publisher><subject>Analysis ; Biomarkers ; Calcitonin ; Cancer ; Carcinoma ; Cell-free DNA ; Circulating biomarker ; Circulating DNA fragmentation ; Circulating DNA methylation ; Correspondence ; Diagnosis ; DNA ; DNA fragmentation ; DNA methylation ; Epigenetics ; Genetic aspects ; Internet ; Medullary thyroid carcinoma ; Methylation ; Mutation ; Neuroendocrine tumors ; Rare tumour ; Remission ; Serum levels ; Size distribution ; Surgery ; Thyroid cancer ; Thyroid carcinoma ; Thyroid diseases ; Trends ; Tumors</subject><ispartof>Biomarker research, 2023-09, Vol.11 (1), p.1-82, Article 82</ispartof><rights>COPYRIGHT 2023 BioMed Central Ltd.</rights><rights>2023. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Yumed Inc. and BioMed Central Ltd., part of Springer Nature 2023</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c526t-7e39dd3d0add0316cc4337bef878719643c2437a55dd19628815fbe0054caf523</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10510276/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2877495081?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793</link.rule.ids></links><search><creatorcontrib>Citarella, Anna</creatorcontrib><creatorcontrib>Besharat, Zein Mersini</creatorcontrib><creatorcontrib>Trocchianesi, Sofia</creatorcontrib><creatorcontrib>Autilio, Tanja Milena</creatorcontrib><creatorcontrib>Verrienti, Antonella</creatorcontrib><creatorcontrib>Catanzaro, Giuseppina</creatorcontrib><creatorcontrib>Splendiani, Elena</creatorcontrib><creatorcontrib>Spinello, Zaira</creatorcontrib><creatorcontrib>Cantara, Silvia</creatorcontrib><creatorcontrib>Zavattari, Patrizia</creatorcontrib><creatorcontrib>Loi, Eleonora</creatorcontrib><creatorcontrib>Romei, Cristina</creatorcontrib><creatorcontrib>Ciampi, Raffaele</creatorcontrib><creatorcontrib>Pezzullo, Luciano</creatorcontrib><creatorcontrib>Castagna, Maria Grazia</creatorcontrib><creatorcontrib>Angeloni, Antonio</creatorcontrib><creatorcontrib>Elisei, Rosella</creatorcontrib><creatorcontrib>Durante, Cosimo</creatorcontrib><creatorcontrib>Po, Agnese</creatorcontrib><creatorcontrib>Ferretti, Elisabetta</creatorcontrib><title>Circulating cell-free DNA (cfDNA) in patients with medullary thyroid carcinoma is characterized by specific fragmentation and methylation changes with diagnostic value</title><title>Biomarker research</title><description>Medullary Thyroid Carcinoma (MTC) is a rare neuroendocrine tumour whose diagnosis includes evaluating calcitonin serum levels, which can present fluctuations unrelated to MTC. Here, we investigated circulating DNA fragmentation and methylation changes as potential biomarkers using ddPCR on cell-free DNA (cfDNA) isolated from the plasma of MTC patients. For cfDNA fragmentation analysis, we investigated the fragment size distribution of a gene family and calculated short fragment fraction (SFF). Methylation analyses evaluated the methylation levels of CG_16698623, a CG dinucleotide in the MGMT gene that we found hypermethylated in MTC tissues by analyzing public databases. The SFF ratio and methylation of CG_16698623 were significantly increased in plasma from MTC patients at diagnosis, and patients with clinical remission or stable disease at follow-up showed no significant SFF difference compared with healthy subjects. Our data support the diagnostic value of cfDNA traits that could enable better management of MTC patients.</description><subject>Analysis</subject><subject>Biomarkers</subject><subject>Calcitonin</subject><subject>Cancer</subject><subject>Carcinoma</subject><subject>Cell-free DNA</subject><subject>Circulating biomarker</subject><subject>Circulating DNA fragmentation</subject><subject>Circulating DNA methylation</subject><subject>Correspondence</subject><subject>Diagnosis</subject><subject>DNA</subject><subject>DNA fragmentation</subject><subject>DNA methylation</subject><subject>Epigenetics</subject><subject>Genetic aspects</subject><subject>Internet</subject><subject>Medullary thyroid carcinoma</subject><subject>Methylation</subject><subject>Mutation</subject><subject>Neuroendocrine tumors</subject><subject>Rare tumour</subject><subject>Remission</subject><subject>Serum levels</subject><subject>Size distribution</subject><subject>Surgery</subject><subject>Thyroid cancer</subject><subject>Thyroid carcinoma</subject><subject>Thyroid diseases</subject><subject>Trends</subject><subject>Tumors</subject><issn>2050-7771</issn><issn>2050-7771</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNptkttu1DAQhiMEotXSF-DKEjftRYrP9l6hquVQqQKJw7Xl9SHrKmsvdlIoL8RrMu1WwCIcWRPb_3zO_Jmue07wKSFavmwcM8l7TFmPsaC054-6Q4oF7pVS5PFf7wfdUWvXGIbWnJDl0-6AKUWlpuqw-3meqptHO6U8IBfGsY81BHTx_gwduwjhBKWMtnAe8tTQtzSt0Sb4eRxtvUXT-raW5JGz1aVcNhalhtzaVuumUNOP4NHqFrVtcCkmh2K1wwY4QCsZ2ewBBYhxt4a8PISHO3yyQy5tgqwbO87hWfck2rGFo4e46L68ef35_F1_9eHt5fnZVe8ElVOvAlt6zzy23mNGpHOcMbUKUSutyFJy5ihnygrhPSyp1kTEVQAHubNRULboLndcX-y12da0gTpNscncb5Q6GFvhq8ZgBBC4kytJFeeMOM3VMrCVZpKKGKUG1qsdazuvwDMHlVc77kH3T3Jam6HcGIIFwVRJIBw_EGr5Ooc2mU1qd3_J5lDmZqiWUgnNmADpi3-k12WuGbwClVJ8KbAmf1SDhQpSjgUudndQc6akWIJbMBfd6X9U8PiwSa7kEBPs7yWc7CWAZgrfp8HOrZnLTx_3tXSndbW0VkP8bQjB5q61za61DbS2uW9tw9kvVCTp4g</recordid><startdate>20230919</startdate><enddate>20230919</enddate><creator>Citarella, Anna</creator><creator>Besharat, Zein Mersini</creator><creator>Trocchianesi, Sofia</creator><creator>Autilio, Tanja Milena</creator><creator>Verrienti, Antonella</creator><creator>Catanzaro, Giuseppina</creator><creator>Splendiani, Elena</creator><creator>Spinello, Zaira</creator><creator>Cantara, Silvia</creator><creator>Zavattari, Patrizia</creator><creator>Loi, Eleonora</creator><creator>Romei, Cristina</creator><creator>Ciampi, Raffaele</creator><creator>Pezzullo, Luciano</creator><creator>Castagna, Maria Grazia</creator><creator>Angeloni, Antonio</creator><creator>Elisei, Rosella</creator><creator>Durante, Cosimo</creator><creator>Po, Agnese</creator><creator>Ferretti, Elisabetta</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><general>BMC</general><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>8FE</scope><scope>8FH</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20230919</creationdate><title>Circulating cell-free DNA (cfDNA) in patients with medullary thyroid carcinoma is characterized by specific fragmentation and methylation changes with diagnostic value</title><author>Citarella, Anna ; Besharat, Zein Mersini ; Trocchianesi, Sofia ; Autilio, Tanja Milena ; Verrienti, Antonella ; Catanzaro, Giuseppina ; Splendiani, Elena ; Spinello, Zaira ; Cantara, Silvia ; Zavattari, Patrizia ; Loi, Eleonora ; Romei, Cristina ; Ciampi, Raffaele ; Pezzullo, Luciano ; Castagna, Maria Grazia ; Angeloni, Antonio ; Elisei, Rosella ; Durante, Cosimo ; Po, Agnese ; Ferretti, Elisabetta</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-7e39dd3d0add0316cc4337bef878719643c2437a55dd19628815fbe0054caf523</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Analysis</topic><topic>Biomarkers</topic><topic>Calcitonin</topic><topic>Cancer</topic><topic>Carcinoma</topic><topic>Cell-free DNA</topic><topic>Circulating biomarker</topic><topic>Circulating DNA fragmentation</topic><topic>Circulating DNA methylation</topic><topic>Correspondence</topic><topic>Diagnosis</topic><topic>DNA</topic><topic>DNA fragmentation</topic><topic>DNA methylation</topic><topic>Epigenetics</topic><topic>Genetic aspects</topic><topic>Internet</topic><topic>Medullary thyroid carcinoma</topic><topic>Methylation</topic><topic>Mutation</topic><topic>Neuroendocrine tumors</topic><topic>Rare tumour</topic><topic>Remission</topic><topic>Serum levels</topic><topic>Size distribution</topic><topic>Surgery</topic><topic>Thyroid cancer</topic><topic>Thyroid carcinoma</topic><topic>Thyroid diseases</topic><topic>Trends</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Citarella, Anna</creatorcontrib><creatorcontrib>Besharat, Zein Mersini</creatorcontrib><creatorcontrib>Trocchianesi, Sofia</creatorcontrib><creatorcontrib>Autilio, Tanja Milena</creatorcontrib><creatorcontrib>Verrienti, Antonella</creatorcontrib><creatorcontrib>Catanzaro, Giuseppina</creatorcontrib><creatorcontrib>Splendiani, Elena</creatorcontrib><creatorcontrib>Spinello, Zaira</creatorcontrib><creatorcontrib>Cantara, Silvia</creatorcontrib><creatorcontrib>Zavattari, Patrizia</creatorcontrib><creatorcontrib>Loi, Eleonora</creatorcontrib><creatorcontrib>Romei, Cristina</creatorcontrib><creatorcontrib>Ciampi, Raffaele</creatorcontrib><creatorcontrib>Pezzullo, Luciano</creatorcontrib><creatorcontrib>Castagna, Maria Grazia</creatorcontrib><creatorcontrib>Angeloni, Antonio</creatorcontrib><creatorcontrib>Elisei, Rosella</creatorcontrib><creatorcontrib>Durante, Cosimo</creatorcontrib><creatorcontrib>Po, Agnese</creatorcontrib><creatorcontrib>Ferretti, Elisabetta</creatorcontrib><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>ProQuest Biological Science Journals</collection><collection>Publicly Available Content Database (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Biomarker research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Citarella, Anna</au><au>Besharat, Zein Mersini</au><au>Trocchianesi, Sofia</au><au>Autilio, Tanja Milena</au><au>Verrienti, Antonella</au><au>Catanzaro, Giuseppina</au><au>Splendiani, Elena</au><au>Spinello, Zaira</au><au>Cantara, Silvia</au><au>Zavattari, Patrizia</au><au>Loi, Eleonora</au><au>Romei, Cristina</au><au>Ciampi, Raffaele</au><au>Pezzullo, Luciano</au><au>Castagna, Maria Grazia</au><au>Angeloni, Antonio</au><au>Elisei, Rosella</au><au>Durante, Cosimo</au><au>Po, Agnese</au><au>Ferretti, Elisabetta</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Circulating cell-free DNA (cfDNA) in patients with medullary thyroid carcinoma is characterized by specific fragmentation and methylation changes with diagnostic value</atitle><jtitle>Biomarker research</jtitle><date>2023-09-19</date><risdate>2023</risdate><volume>11</volume><issue>1</issue><spage>1</spage><epage>82</epage><pages>1-82</pages><artnum>82</artnum><issn>2050-7771</issn><eissn>2050-7771</eissn><abstract>Medullary Thyroid Carcinoma (MTC) is a rare neuroendocrine tumour whose diagnosis includes evaluating calcitonin serum levels, which can present fluctuations unrelated to MTC. Here, we investigated circulating DNA fragmentation and methylation changes as potential biomarkers using ddPCR on cell-free DNA (cfDNA) isolated from the plasma of MTC patients. For cfDNA fragmentation analysis, we investigated the fragment size distribution of a gene family and calculated short fragment fraction (SFF). Methylation analyses evaluated the methylation levels of CG_16698623, a CG dinucleotide in the MGMT gene that we found hypermethylated in MTC tissues by analyzing public databases. The SFF ratio and methylation of CG_16698623 were significantly increased in plasma from MTC patients at diagnosis, and patients with clinical remission or stable disease at follow-up showed no significant SFF difference compared with healthy subjects. Our data support the diagnostic value of cfDNA traits that could enable better management of MTC patients.</abstract><cop>London</cop><pub>BioMed Central Ltd</pub><pmid>37726827</pmid><doi>10.1186/s40364-023-00522-4</doi><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 2050-7771
ispartof Biomarker research, 2023-09, Vol.11 (1), p.1-82, Article 82
issn 2050-7771
2050-7771
language eng
recordid cdi_doaj_primary_oai_doaj_org_article_5d194c6b6274431c8479e3b83625ff68
source Open Access: PubMed Central; Publicly Available Content Database (Proquest) (PQ_SDU_P3)
subjects Analysis
Biomarkers
Calcitonin
Cancer
Carcinoma
Cell-free DNA
Circulating biomarker
Circulating DNA fragmentation
Circulating DNA methylation
Correspondence
Diagnosis
DNA
DNA fragmentation
DNA methylation
Epigenetics
Genetic aspects
Internet
Medullary thyroid carcinoma
Methylation
Mutation
Neuroendocrine tumors
Rare tumour
Remission
Serum levels
Size distribution
Surgery
Thyroid cancer
Thyroid carcinoma
Thyroid diseases
Trends
Tumors
title Circulating cell-free DNA (cfDNA) in patients with medullary thyroid carcinoma is characterized by specific fragmentation and methylation changes with diagnostic value
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T17%3A53%3A43IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Circulating%20cell-free%20DNA%20(cfDNA)%20in%20patients%20with%20medullary%20thyroid%20carcinoma%20is%20characterized%20by%20specific%20fragmentation%20and%20methylation%20changes%20with%20diagnostic%20value&rft.jtitle=Biomarker%20research&rft.au=Citarella,%20Anna&rft.date=2023-09-19&rft.volume=11&rft.issue=1&rft.spage=1&rft.epage=82&rft.pages=1-82&rft.artnum=82&rft.issn=2050-7771&rft.eissn=2050-7771&rft_id=info:doi/10.1186/s40364-023-00522-4&rft_dat=%3Cgale_doaj_%3EA765943394%3C/gale_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c526t-7e39dd3d0add0316cc4337bef878719643c2437a55dd19628815fbe0054caf523%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2877495081&rft_id=info:pmid/37726827&rft_galeid=A765943394&rfr_iscdi=true