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LncRNA XIST promotes bladder cancer progression by modulating miR-129-5p/TNFSF10 axis
Background The differential expression, biological function, and ceRNA regulatory mechanism of lncRNA XIST in bladder cancer (BC) were investigated, and its clinical values for the early diagnosis of bladder cancer patients were elucidated. Methods qRT-PCR was employed to detect the expression patte...
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| Published in: | Discover. Oncology 2024-03, Vol.15 (1), p.65-65, Article 65 |
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| container_title | Discover. Oncology |
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| creator | Kong, Yu-Lin Wang, Hui-Dan Gao, Meng Rong, Sheng-Zhong Li, Xiao-Xia |
| description | Background
The differential expression, biological function, and ceRNA regulatory mechanism of lncRNA XIST in bladder cancer (BC) were investigated, and its clinical values for the early diagnosis of bladder cancer patients were elucidated.
Methods
qRT-PCR was employed to detect the expression patterns of lncRNA XIST, miR-129-5p and TNFSF10. The biological functions were measured by CCK8 assay, wound healing assay and transwell assay. Bioinformatics analysis and Dual-Luciferase reporter assay were employed to evaluate the interactions between the lncRNA XIST, miR-129-5p and TNFSF10.
Results
LncRNA XIST and TNFSF10 were highly expressed and miR-129-5p was low expressed (
P
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| doi_str_mv | 10.1007/s12672-024-00910-8 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_5d28fae2d197438a95f4a1fd3e1e9235</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_5d28fae2d197438a95f4a1fd3e1e9235</doaj_id><sourcerecordid>2938140688</sourcerecordid><originalsourceid>FETCH-LOGICAL-c492t-d8abf0871abbd00873dbae1dcb8df08b244eb04d00a570679b3ea9115cb37de23</originalsourceid><addsrcrecordid>eNp9Uk1r3DAQFaUhCdv8gRyKoZde3Iw-bMmnEkK2XVhSSDbQm9CXXS-2tZXs0vz7atdpmuTQ0wzz3rwZjR5C5xg-YQB-ETEpOcmBsBygwpCLN-iUcAp5CRi_fZafoLMYtwBACkwpFMfohArGSlLwU3S_HsztzWX2fXW3yXbB9350MdOdstaFzKjBpJDqTXAxtn7I9EPWezt1amyHJuvb2xyTKi92F5ub5d0SQ6Z-t_EdOqpVF93ZY1yg--X15uprvv72ZXV1uc4Nq8iYW6F0DYJjpbWFlFCrlcPWaGFTXRPGnAaWIFVwKHmlqVMVxoXRlFtH6AKtZl3r1VbuQtur8CC9auWh4EMjVRhb0zlZWCJq5YjFFWdUqKqomcK1pQ67itAiaX2etXaT7p01bhiD6l6IvkSG9ods_C-J0_k5T6ddoI-PCsH_nFwcZd9G47pODc5PUZKKCiI40P3iH15Rt34KQ7rVgYUZlEIkFplZJvgYg6uftsEg9y6QswtkcoE8uEDum94_f8dTy98_TwQ6E2KChsaFf7P_I_sH9MS7Pg</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2938140688</pqid></control><display><type>article</type><title>LncRNA XIST promotes bladder cancer progression by modulating miR-129-5p/TNFSF10 axis</title><source>Open Access: PubMed Central</source><source>Publicly Available Content Database</source><creator>Kong, Yu-Lin ; Wang, Hui-Dan ; Gao, Meng ; Rong, Sheng-Zhong ; Li, Xiao-Xia</creator><creatorcontrib>Kong, Yu-Lin ; Wang, Hui-Dan ; Gao, Meng ; Rong, Sheng-Zhong ; Li, Xiao-Xia</creatorcontrib><description>Background
The differential expression, biological function, and ceRNA regulatory mechanism of lncRNA XIST in bladder cancer (BC) were investigated, and its clinical values for the early diagnosis of bladder cancer patients were elucidated.
Methods
qRT-PCR was employed to detect the expression patterns of lncRNA XIST, miR-129-5p and TNFSF10. The biological functions were measured by CCK8 assay, wound healing assay and transwell assay. Bioinformatics analysis and Dual-Luciferase reporter assay were employed to evaluate the interactions between the lncRNA XIST, miR-129-5p and TNFSF10.
Results
LncRNA XIST and TNFSF10 were highly expressed and miR-129-5p was low expressed (
P
< 0.05) in bladder cancer cell line. The depletion of lncRNA XIST inhibited BC proliferation, migration and invasion. Mechanistically, lncRNA XIST could sponge miR-129-5p to regulate TNFSF10 expression in bladder cancer. Furthermore, compared with adjacent tissues, lncRNA XIST and miR-129-5p were lowly expressed (
P
< 0.01) in bladder cancer tissues, and TNFSF10 was highly expressed (
P
< 0.001). miR-129-5p and TNFSF10 were associated with the risk of bladder cancer (
P
< 0.05); the difference in AUC values for the diagnosis of bladder cancer by lncRNA XIST (AUC = 0.739), miR-129-5p (AUC = 0.850) and TNFSF10 (AUC = 0.753) was statistically significant (
P
< 0.01), and the three genes combined AUC was 0.900, 95%CI was 0.842–0.958 with a sensitivity of 83.3% and specificity of 86.7%.
Conclusion
XIST, an elevated lncRNA in bladder cancer, inhibition of which could suppress the progression of BC. LncRNA XIST and miR-129-5p could form ceRNA to regulate the expression of TNFSF10.</description><identifier>ISSN: 2730-6011</identifier><identifier>EISSN: 2730-6011</identifier><identifier>DOI: 10.1007/s12672-024-00910-8</identifier><identifier>PMID: 38446257</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Binding sites ; Bioinformatics ; Biomarkers ; Bladder cancer ; Cancer Research ; Cell growth ; Chemotherapy ; Genes ; Genomes ; Internal Medicine ; Medicine ; Medicine & Public Health ; Metastasis ; MicroRNAs ; Molecular Medicine ; Oncology ; Pathogenesis ; Radiation therapy ; Radiotherapy ; Statistical analysis ; Surgical Oncology ; Tumors ; X chromosomes</subject><ispartof>Discover. Oncology, 2024-03, Vol.15 (1), p.65-65, Article 65</ispartof><rights>The Author(s) 2024</rights><rights>2024. The Author(s).</rights><rights>The Author(s) 2024. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c492t-d8abf0871abbd00873dbae1dcb8df08b244eb04d00a570679b3ea9115cb37de23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2938140688/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2938140688?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,25732,27903,27904,36991,36992,44569,53769,53771,74872</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38446257$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kong, Yu-Lin</creatorcontrib><creatorcontrib>Wang, Hui-Dan</creatorcontrib><creatorcontrib>Gao, Meng</creatorcontrib><creatorcontrib>Rong, Sheng-Zhong</creatorcontrib><creatorcontrib>Li, Xiao-Xia</creatorcontrib><title>LncRNA XIST promotes bladder cancer progression by modulating miR-129-5p/TNFSF10 axis</title><title>Discover. Oncology</title><addtitle>Discov Onc</addtitle><addtitle>Discov Oncol</addtitle><description>Background
The differential expression, biological function, and ceRNA regulatory mechanism of lncRNA XIST in bladder cancer (BC) were investigated, and its clinical values for the early diagnosis of bladder cancer patients were elucidated.
Methods
qRT-PCR was employed to detect the expression patterns of lncRNA XIST, miR-129-5p and TNFSF10. The biological functions were measured by CCK8 assay, wound healing assay and transwell assay. Bioinformatics analysis and Dual-Luciferase reporter assay were employed to evaluate the interactions between the lncRNA XIST, miR-129-5p and TNFSF10.
Results
LncRNA XIST and TNFSF10 were highly expressed and miR-129-5p was low expressed (
P
< 0.05) in bladder cancer cell line. The depletion of lncRNA XIST inhibited BC proliferation, migration and invasion. Mechanistically, lncRNA XIST could sponge miR-129-5p to regulate TNFSF10 expression in bladder cancer. Furthermore, compared with adjacent tissues, lncRNA XIST and miR-129-5p were lowly expressed (
P
< 0.01) in bladder cancer tissues, and TNFSF10 was highly expressed (
P
< 0.001). miR-129-5p and TNFSF10 were associated with the risk of bladder cancer (
P
< 0.05); the difference in AUC values for the diagnosis of bladder cancer by lncRNA XIST (AUC = 0.739), miR-129-5p (AUC = 0.850) and TNFSF10 (AUC = 0.753) was statistically significant (
P
< 0.01), and the three genes combined AUC was 0.900, 95%CI was 0.842–0.958 with a sensitivity of 83.3% and specificity of 86.7%.
Conclusion
XIST, an elevated lncRNA in bladder cancer, inhibition of which could suppress the progression of BC. LncRNA XIST and miR-129-5p could form ceRNA to regulate the expression of TNFSF10.</description><subject>Binding sites</subject><subject>Bioinformatics</subject><subject>Biomarkers</subject><subject>Bladder cancer</subject><subject>Cancer Research</subject><subject>Cell growth</subject><subject>Chemotherapy</subject><subject>Genes</subject><subject>Genomes</subject><subject>Internal Medicine</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Metastasis</subject><subject>MicroRNAs</subject><subject>Molecular Medicine</subject><subject>Oncology</subject><subject>Pathogenesis</subject><subject>Radiation therapy</subject><subject>Radiotherapy</subject><subject>Statistical analysis</subject><subject>Surgical Oncology</subject><subject>Tumors</subject><subject>X chromosomes</subject><issn>2730-6011</issn><issn>2730-6011</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNp9Uk1r3DAQFaUhCdv8gRyKoZde3Iw-bMmnEkK2XVhSSDbQm9CXXS-2tZXs0vz7atdpmuTQ0wzz3rwZjR5C5xg-YQB-ETEpOcmBsBygwpCLN-iUcAp5CRi_fZafoLMYtwBACkwpFMfohArGSlLwU3S_HsztzWX2fXW3yXbB9350MdOdstaFzKjBpJDqTXAxtn7I9EPWezt1amyHJuvb2xyTKi92F5ub5d0SQ6Z-t_EdOqpVF93ZY1yg--X15uprvv72ZXV1uc4Nq8iYW6F0DYJjpbWFlFCrlcPWaGFTXRPGnAaWIFVwKHmlqVMVxoXRlFtH6AKtZl3r1VbuQtur8CC9auWh4EMjVRhb0zlZWCJq5YjFFWdUqKqomcK1pQ67itAiaX2etXaT7p01bhiD6l6IvkSG9ods_C-J0_k5T6ddoI-PCsH_nFwcZd9G47pODc5PUZKKCiI40P3iH15Rt34KQ7rVgYUZlEIkFplZJvgYg6uftsEg9y6QswtkcoE8uEDum94_f8dTy98_TwQ6E2KChsaFf7P_I_sH9MS7Pg</recordid><startdate>20240306</startdate><enddate>20240306</enddate><creator>Kong, Yu-Lin</creator><creator>Wang, Hui-Dan</creator><creator>Gao, Meng</creator><creator>Rong, Sheng-Zhong</creator><creator>Li, Xiao-Xia</creator><general>Springer US</general><general>Springer Nature B.V</general><general>Springer</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>NAPCQ</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20240306</creationdate><title>LncRNA XIST promotes bladder cancer progression by modulating miR-129-5p/TNFSF10 axis</title><author>Kong, Yu-Lin ; Wang, Hui-Dan ; Gao, Meng ; Rong, Sheng-Zhong ; Li, Xiao-Xia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c492t-d8abf0871abbd00873dbae1dcb8df08b244eb04d00a570679b3ea9115cb37de23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Binding sites</topic><topic>Bioinformatics</topic><topic>Biomarkers</topic><topic>Bladder cancer</topic><topic>Cancer Research</topic><topic>Cell growth</topic><topic>Chemotherapy</topic><topic>Genes</topic><topic>Genomes</topic><topic>Internal Medicine</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Metastasis</topic><topic>MicroRNAs</topic><topic>Molecular Medicine</topic><topic>Oncology</topic><topic>Pathogenesis</topic><topic>Radiation therapy</topic><topic>Radiotherapy</topic><topic>Statistical analysis</topic><topic>Surgical Oncology</topic><topic>Tumors</topic><topic>X chromosomes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kong, Yu-Lin</creatorcontrib><creatorcontrib>Wang, Hui-Dan</creatorcontrib><creatorcontrib>Gao, Meng</creatorcontrib><creatorcontrib>Rong, Sheng-Zhong</creatorcontrib><creatorcontrib>Li, Xiao-Xia</creatorcontrib><collection>Springer_OA刊</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Nursing & Allied Health Premium</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Discover. Oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kong, Yu-Lin</au><au>Wang, Hui-Dan</au><au>Gao, Meng</au><au>Rong, Sheng-Zhong</au><au>Li, Xiao-Xia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>LncRNA XIST promotes bladder cancer progression by modulating miR-129-5p/TNFSF10 axis</atitle><jtitle>Discover. Oncology</jtitle><stitle>Discov Onc</stitle><addtitle>Discov Oncol</addtitle><date>2024-03-06</date><risdate>2024</risdate><volume>15</volume><issue>1</issue><spage>65</spage><epage>65</epage><pages>65-65</pages><artnum>65</artnum><issn>2730-6011</issn><eissn>2730-6011</eissn><abstract>Background
The differential expression, biological function, and ceRNA regulatory mechanism of lncRNA XIST in bladder cancer (BC) were investigated, and its clinical values for the early diagnosis of bladder cancer patients were elucidated.
Methods
qRT-PCR was employed to detect the expression patterns of lncRNA XIST, miR-129-5p and TNFSF10. The biological functions were measured by CCK8 assay, wound healing assay and transwell assay. Bioinformatics analysis and Dual-Luciferase reporter assay were employed to evaluate the interactions between the lncRNA XIST, miR-129-5p and TNFSF10.
Results
LncRNA XIST and TNFSF10 were highly expressed and miR-129-5p was low expressed (
P
< 0.05) in bladder cancer cell line. The depletion of lncRNA XIST inhibited BC proliferation, migration and invasion. Mechanistically, lncRNA XIST could sponge miR-129-5p to regulate TNFSF10 expression in bladder cancer. Furthermore, compared with adjacent tissues, lncRNA XIST and miR-129-5p were lowly expressed (
P
< 0.01) in bladder cancer tissues, and TNFSF10 was highly expressed (
P
< 0.001). miR-129-5p and TNFSF10 were associated with the risk of bladder cancer (
P
< 0.05); the difference in AUC values for the diagnosis of bladder cancer by lncRNA XIST (AUC = 0.739), miR-129-5p (AUC = 0.850) and TNFSF10 (AUC = 0.753) was statistically significant (
P
< 0.01), and the three genes combined AUC was 0.900, 95%CI was 0.842–0.958 with a sensitivity of 83.3% and specificity of 86.7%.
Conclusion
XIST, an elevated lncRNA in bladder cancer, inhibition of which could suppress the progression of BC. LncRNA XIST and miR-129-5p could form ceRNA to regulate the expression of TNFSF10.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>38446257</pmid><doi>10.1007/s12672-024-00910-8</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| source | Open Access: PubMed Central; Publicly Available Content Database |
| subjects | Binding sites Bioinformatics Biomarkers Bladder cancer Cancer Research Cell growth Chemotherapy Genes Genomes Internal Medicine Medicine Medicine & Public Health Metastasis MicroRNAs Molecular Medicine Oncology Pathogenesis Radiation therapy Radiotherapy Statistical analysis Surgical Oncology Tumors X chromosomes |
| title | LncRNA XIST promotes bladder cancer progression by modulating miR-129-5p/TNFSF10 axis |
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