MEG source imaging detects optogenetically-induced activity in cortical and subcortical networks

• Published in: Nature communications 2021-09, Vol.12 (1), p.5259-5259, Article 5259
• Main Authors: Alberto, Gregory E., Stapleton-Kotloski, Jennifer R., Klorig, David C., Rogers, Emily R., Constantinidis, Christos, Daunais, James B., Godwin, Dwayne W.
• Format: Article
• Language: English
• Subjects: 13/44; 14/35; 59/57; 631/1647/2253; 631/1647/245/1887; 631/378/3920; 9/26; 9/30; Animals; Bacterial Proteins - genetics; Brain; Brain - diagnostic imaging; Brain - physiology; Brain mapping; Chlorocebus aethiops; Cortex (temporal); Evoked Potentials - physiology; Female; Functional Neuroimaging - methods; High resolution; Humanities and Social Sciences; Image resolution; Localization; Luminescent Proteins - genetics; Magnetoencephalography; Magnetoencephalography - methods; Microscopy, Confocal; Models, Neurological; multidisciplinary; Nerve Net; Neuroimaging; Optogenetics - methods; Primates; Science; Science (multidisciplinary); Signal Processing, Computer-Assisted; Spatial discrimination; Spatial resolution; Thalamus
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-021-25481-y

Magnetoencephalography measures neuromagnetic activity with high temporal, and theoretically, high spatial resolution. We developed an experimental platform combining MEG-compatible optogenetic techniques in nonhuman primates for use as a functional brain-mapping platform. Here we show localization of optogenetically evoked signals to known sources in the superficial arcuate sulcus of cortex and in CA3 of hippocampus at a resolution of 750 µm 3 . We detect activation in subcortical, thalamic, and extended temporal structures, conforming to known anatomical and functional brain networks associated with the respective sites of stimulation. This demonstrates that high-resolution localization of experimentally produced deep sources is possible within an intact brain. This approach is suitable for exploring causal relationships between discrete brain regions through precise optogenetic control and simultaneous whole brain MEG recording with high-resolution magnetic source imaging (MSI). Identifying causal interactions between brain regions is important to understand its computations. Here the authors present optoMEG, a platform for combining optogenetic techniques with high-resolution magnetic source imaging in nonhuman primates to map network activation.