Rapid detection of Van genes in rectal swabs by real time PCR in Southern Brazil

Laboratory-based surveillance is an important component in the control of vancomycin resistant enterococci (VRE). The study aimed to evaluate real-time polymerase chain reaction (RT-PCR) (genes vanA-vanB) for VRE detection on 115 swabs from patients included in a surveillance program. Sensitivity of...

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Published in:Revista da Sociedade Brasileira de Medicina Tropical 2011-10, Vol.44 (5), p.631-632
Main Authors: Cantarelli, Vlademir, Cavalcante, Bianca, Pilger, Diogo André, Souza, Fabiana, Dias, Cícero Gomes, Brodt, Teresa, Cantarelli, Maritza, Secchi, Carina, d'Azevedo, Pedro Alves
Format: Article
Language:English
Subjects:
Bacterial Proteins - genetics
Carbon-Oxygen Ligases - genetics
Enterococcus - genetics
Enterococcus - isolation & purification
Enterococos resistentes à vancomicina
Genes vanA-vanB
Gram-Positive Bacterial Infections - diagnosis
Gram-Positive Bacterial Infections - microbiology
Humans
Predictive Value of Tests
Real-Time Polymerase Chain Reaction - methods
Rectum - microbiology
RT-PCR
Sensitivity and Specificity
TROPICAL MEDICINE
Vancomycin Resistance - genetics
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Summary:Laboratory-based surveillance is an important component in the control of vancomycin resistant enterococci (VRE). The study aimed to evaluate real-time polymerase chain reaction (RT-PCR) (genes vanA-vanB) for VRE detection on 115 swabs from patients included in a surveillance program. Sensitivity of RT-PCR was similar to primary culture (75% and 79.5%, respectively) when compared to broth enriched culture, whereas specificity was 83.1%. RT-PCR provides same day results, however it showed low sensitivity for VRE detection.
ISSN:0037-8682
1678-9849
1678-9849
0037-8682
DOI:10.1590/S0037-86822011000500021