Early evaluation of salt-stress tolerance of new released olive cultivars based on physiological and biomass allocation indicators

• Published in: Journal of agriculture and food research 2024-12, Vol.18, p.101503, Article 101503
• Main Authors: Elloumi, Olfa, Amar, Fathi Ben, Trigui, Ahmed, Gargouri, Kamel, Ghrab, Mohamed
• Format: Article
• Language: English
• Subjects: Early salt-stress evaluation; New cultivars; Olea europaea; Salt-stress indicators
• ISSN: 2666-1543; 2666-1543
• DOI: 10.1016/j.jafr.2024.101503

The evaluation of salt-tolerant olive tree genotypes released from breeding program has become intrinsic in order to develop sustainable agriculture in these regions. The current study aims to i) evaluate the tolerance of two new released olive cultivars (Zeitoun Ennour and Zeitoun Ennwader), issued from a Tunisian breeding program in comparison with the main olive cultivar ‘Chemlali Sfax’, and ii) identify suitable salt-tolerance indicators for this evaluation. The trial was conducted under controlled conditions in a greenhouse, for six months, and plants were irrigated with half-strength Hoagland nutrient solution containing NaCl at various levels (0, 75, 150 and 225 mM). Vegetative growth, biomass allocation and biochemical parameters were considered for the assessment of salt-tolerant capacity. Results revealed that growth and biomass should be considered as useful indicators for early evaluation method of salt-stress tolerance. ‘Chemlali Sfax’ displayed a gradual significant decrease of vegetative growth with increasing salinity. The reduction of shoot elongation and trunk diameter was most evidently at 150 mM with 43.2 % and 80.8 %, respectively. However, the new released cultivars showed unaffected vegetative growth, despite a slight decrease at the salt level of 225 mM. Moreover, results demonstrated the importance of certain physiological and biochemical indicators such as ions contents. The new released cultivars maintained a high K+/Na+ ratio at moderate salinity then a significant decrease occurred at 150 mM. In conclusion, vegetative growth, biomass allocation and K+/Na+ ratio seemed to be the suitable salt-tolerance indicators for early evaluation. Based on these indicators, ‘Zeitoun Ennour’ seems to be the most salt tolerant genotype and ‘Zeitoun Ennwader’ proves to have a similar salt tolerant capacity as ‘Chemlali Sfax’, which is a valuable information for olive growers in arid region. [Display omitted] •Olive growing is a valuable cultivation in arid areas and saline water irrigation.•Vegetative growth, biomass allocation and K+/Na+ ratio should be considered as the most reliable indicators for early salt-stress evaluation of young olive tree independently of the genetic background.•Early, easy and quickly method for evaluating olive salt-stress.