PbrWRKY62-PbrADC1 module involves in superficial scald development of Pyrus bretschneideri Rehd.fruit via regulating putrescine biosynthesis

Putrescine plays a role in superficial scald development during the cold storage of pear fruit. However, the molecular mechanism behind this phenomenon has not been un-fully clarified until recently. In this study, a conjoint analysis of metabolites and gene expression profiles in the putrescine-met...

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Bibliographic Details
Published in:Molecular horticulture 2024-02, Vol.4 (1), p.6-6, Article 6
Main Authors: Zhang, Xu, Zhu, Lijuan, Qian, Ming, Jiang, Li, Gu, Peng, Jia, Luting, Qian, Chunlu, Luo, Weiqi, Ma, Min, Wu, Zhangfei, Qiao, Xin, Wang, Libin, Zhang, Shaoling
Format: Article
Language:English
Subjects:
Abiotic stress
Biosynthesis
Cold storage
Fruits
Gene expression
Genes
Genomes
Metabolism
Metabolomics
P. bretschneideri Rehd
PbrWRKY62-PbrADC1 module
Polyamines
Putrescine biosynthesis
Superficial scald development
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Summary:Putrescine plays a role in superficial scald development during the cold storage of pear fruit. However, the molecular mechanism behind this phenomenon has not been un-fully clarified until recently. In this study, a conjoint analysis of metabolites and gene expression profiles in the putrescine-metabolic pathway of P. bretschneideri Rehd. fruit followed by experimental validation revealed that PbrADC1, forming a homodimer in the chloroplast, was involved in putrescine biosynthesis and thus fruit chilling resistance. Additionally, the substrate-binding residue Cys in PbrADC1, whose activity was modified by H O , played a crucial role in arginine decarboxylation into agmatine. Through a combined analysis of the distribution of cis-acting elements in the PbrADC1 promoter as well as the expression profiles of related transcription factors (TFs), several TFs were identified as upstream regulators of PbrADC1 gene. Further investigation revealed that the nuclear PbrWRKY62 could directly bind to the W-box elements in the PbrADC1 promoter, activate its expression, enhance putrescine accumulation, and thus increase fruit chilling tolerance. In conclusion, our results suggest that the PbrWRKY62-PbrADC1 module is involved in the development of superficial scald in P. bretschneideri Rehd. fruit via regulating putrescine biosynthesis. Consequently, these findings could serve as valuable genetic resources for breeding scald-resistant pear fruit.
ISSN:2730-9401
2730-9401
DOI:10.1186/s43897-024-00081-8