Distinct In Vitro T-Helper 17 Differentiation Capacity of Peripheral Naive T Cells in Rheumatoid and Psoriatic Arthritis

The T-helper 17 (Th17) cells have a prominent role in inflammation as well as in bone and join destruction in both rheumatoid and psoriatic arthritis (RA and PsA). Here, we studied Th17 cell differentiation in RA and PsA. Blood samples from healthy donors, RA and PsA patients were collected. CD45RO...

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Published in:Frontiers in immunology 2018-04, Vol.9, p.606-606
Main Authors: Baricza, Eszter, Marton, Nikolett, Királyhidi, Panna, Kovács, Orsolya Tünde, Kovácsné Székely, Ilona, Lajkó, Eszter, Kőhidai, Lászó, Rojkovich, Bernadett, Érsek, Barbara, Buzás, Edit Irén, Nagy, György
Format: Article
Language:English
Subjects:
Arthritis, Psoriatic - immunology
Arthritis, Rheumatoid - immunology
Blood Circulation
Cell Differentiation
Cells, Cultured
Flow Cytometry
Humans
Immunity - genetics
Immunologic Memory
Immunology
interleukin-17A
Interleukin-22
Interleukins - genetics
Interleukins - metabolism
Leukocyte Common Antigens - metabolism
Nuclear Receptor Subfamily 1, Group F, Member 3 - genetics
psoriatic arthritis
Receptors, CCR6 - genetics
rheumatoid arthritis
T-Box Domain Proteins - genetics
T-helper 17 differentiation
Th17 Cells - physiology
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Summary:The T-helper 17 (Th17) cells have a prominent role in inflammation as well as in bone and join destruction in both rheumatoid and psoriatic arthritis (RA and PsA). Here, we studied Th17 cell differentiation in RA and PsA. Blood samples from healthy donors, RA and PsA patients were collected. CD45RO (naive) and CD45RO (memory) T cells were isolated from peripherial blood mononuclear cell by magnetic separation. Naive T cells were stimulated with anti-CD3, anti-CD28, and goat anti-mouse IgG antibodies and treated with transforming grow factor beta, interleukin (IL)-6, IL-1 , and IL-23 cytokines and also with anti-IL-4 antibody. IL-17A and IL-22 production were measured by enzyme linked immunosorbent assay, , and ( ) expression were analyzed by quantitative polymerase chain reaction and flow cytometry. C-C chemokine receptor 6 (CCR6), CCR4, and C-X-C motif chemokine receptor 3 expression were determined by flow cytometry. Cell viability was monitored by impedance-based cell analyzer (CASY-TT). , CCR6, and CCR4 expression of memory T cells of healthy individuals (but not RA or PsA patients) were increased (  
ISSN:1664-3224
1664-3224
DOI:10.3389/fimmu.2018.00606