The DPA-derivative 11S, 17S-dihydroxy 7,9,13,15,19 (Z,E,Z,E,Z)-docosapentaenoic acid inhibits IL-6 production by inhibiting ROS production and ERK/NF-κB pathway in keratinocytes HaCaT stimulated with a fine dust PM10

• Published in: Ecotoxicology and environmental safety 2022-03, Vol.232, p.113252-113252, Article 113252
• Main Authors: Choi, Myoung-Kwon, Kim, Jinju, Park, Hyo-Min, Lim, Chae-Min, Pham, Thu-Huyen, Shin, Ha Youn, Kim, Seong-Eun, Oh, Deok-Kun, Yoon, Do-Young
• Format: Article
• Language: English
• Subjects: DoPE; HaCaT cells; MAPK; PM10; ROS; Specialized pro-resolving mediator
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2022.113252

11 S, 17S-dihydroxy 7,9,13,15,19 (Z,E,Z,E,Z)-docosapentaenoic acid (DoPE) is a derivative of docosapentaenoic acid, a specialized pro-resolving mediator of inflammation such as lipoxins, resolvins, maresins, and protectins. PM10 is a fine dust particle that induces oxidative stress, DNA damage, inflammation, aging, and cancer. The anti-inflammatory mechanism of DoPE, however, has not yet been elucidated. In these studies, we investigated whether DoPE has anti-inflammatory effects in human keratinocyte HaCaT cells. We demonstrated that DoPE suppressed PM10-induced expressions of IL-6 mRNA and protein in human HaCaT keratinocytes. We also investigated the modulating effects of DoPE on reactive oxygen species (ROS) and mitogen-activated protein kinase (MAPK). ROS production, extracellular signal regulated kinase (ERK) phosphorylation, and translocation of nuclear factor-kappa B (NF-kB) p65 and NF-kB activity were suppressed by DoPE in PM10-stimulated HaCaT cells. Collectively, our results demonstrated that DoPE inhibited IL-6 expression by reducing ROS generation, suppressing ERK phosphorylation, and inhibiting translocation of NF-kB p65 and NF-kB activity in PM10-stimulated HaCaT cells, suggesting that DoPE can be useful for the resolution of the inflammation caused by IL-6. [Display omitted] •PM10 inhibits the expression of ROS scavenger genes, resulting in ROS generation.•DoPE decreases ROS production by recovering the expression of ROS scavenger genes.•DoPE attenuates ROS induced p-ERK/NF-κB and NF-κB promoter activity.•DoPE inhibits IL-6 production via attenuating ERK/NF-κB and IL-6 promoter activity.