Comprehensive Profiling of Surface Gangliosides Extracted from Various Cell Lines by LC-MS/MS

Gangliosides act as a surface marker at the outer cellular membrane and play key roles in cancer cell invasion and metastasis. Despite the biological importance of gangliosides, they have been still poorly characterized due to the lack of effective analytical tools. Herein, we performed molecular pr...

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Bibliographic Details
Published in:Cells (Basel, Switzerland) Switzerland), 2019-10, Vol.8 (11), p.1323
Main Authors: Lee, Jua, Hwang, Heeyoun, Kim, Sumin, Hwang, Jaeyun, Yoon, Jaekyung, Yin, Dongtan, Choi, Sun Il, Kim, Yun-Hee, Kim, Yong-Sam, An, Hyun Joo
Format: Article
Language:English
Subjects:
Biomarkers
Breast cancer
Cancer
cancer cell
Cell culture
Cell membranes
Cell surface
Ceramide
Clinical trials
ganglioside
Gangliosides
Glycoproteins
glycosylation
lc-ms/ms
Lung cancer
Medical diagnosis
Medical research
Melanoma
Metastases
Metastasis
Neuroblastoma
Pancreas
Pancreatic cancer
Surface markers
Telomerase reverse transcriptase
Tumor cell lines
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Summary:Gangliosides act as a surface marker at the outer cellular membrane and play key roles in cancer cell invasion and metastasis. Despite the biological importance of gangliosides, they have been still poorly characterized due to the lack of effective analytical tools. Herein, we performed molecular profiling and structural elucidation of intact gangliosides in various cell lines including CFPAC1, A549, NCI-H358, MCF7, and Caski. We identified and quantified a total of 76 gangliosides on cell membrane using C18 LC-MS/MS. Gangliosides found in each cell line exhibited high complexity and diversity both qualitatively and quantitatively. The most abundant species was GM3(d34:1) in CFPAC1, NCI-H358, and MCF7, while GM2(d34:1) and GM1(d34:1) were major components in A549 and Caski, respectively. Notably, glycan moieties showed more diversity between cancer cell lines than ceramide moieties. In addition, noncancerous pancreatic cell line (hTERT/HPNE) could be distinguished by gangliosides containing different levels of sialic acid compared with cancerous pancreatic cell line (CFPAC1). These results clearly demonstrated the feasibility of our analytical platform to comprehensive profile of cell surface gangliosides for identifying cell types and subgrouping cancer cell types.
ISSN:2073-4409
2073-4409
DOI:10.3390/cells8111323