The circular RNA circZFR phosphorylates Rb promoting cervical cancer progression by regulating the SSBP1/CDK2/cyclin E1 complex

As a novel type of non-coding RNA, circular RNAs (circRNAs) play a critical role in the initiation and development of various diseases, including cancer. However, the exact function of circRNAs in human cervical cancer remains largely unknown. We identified the circRNA signature of upregulated circR...

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Published in:Journal of experimental & clinical cancer research 2021-01, Vol.40 (1), p.48-48, Article 48
Main Authors: Zhou, Mingyi, Yang, Zhuo, Wang, Danbo, Chen, Peng, Zhang, Yong
Format: Article
Language:English
Subjects:
Antibodies
Biomarkers
Cell cycle
Cell cycle regulation
Cell growth
Cervical cancer
Chemotherapy
Circular RNAs
Development and progression
Gene expression
Mass spectrometry
MicroRNAs
Phosphorylation
Protein binding
Proteins
Radiation therapy
RNA
RNA binding protein
RNA Immunoprecipitation
Software
Transcription factors
Tumors
Wound healing
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cited_by cdi_FETCH-LOGICAL-c594t-db51460a9e5f877b301baf4a25d6d8dd721b18c00aa35f340150963ac65841c83
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creator Zhou, Mingyi
Yang, Zhuo
Wang, Danbo
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description As a novel type of non-coding RNA, circular RNAs (circRNAs) play a critical role in the initiation and development of various diseases, including cancer. However, the exact function of circRNAs in human cervical cancer remains largely unknown. We identified the circRNA signature of upregulated circRNAs between cervical cancer and paired adjacent normal tissues. Using two different cohorts and GEO database, a total of six upregulated circRNAs were identified with a fold change > 2, and P
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However, the exact function of circRNAs in human cervical cancer remains largely unknown. We identified the circRNA signature of upregulated circRNAs between cervical cancer and paired adjacent normal tissues. Using two different cohorts and GEO database, a total of six upregulated circRNAs were identified with a fold change &gt; 2, and P &lt; 0.05. Among these six circRNAs, hsa_circ_0072088 (circZFR) was the only exonic circRNA significantly overexpressed in cervical cancer. Functional experiments were performed to investigate the biological function of circZFR. CircRNA pull-down, circRNA immunoprecipitation (circRIP) and Co-immunoprecipitation (Co-IP) assays were executed to investigate the molecular mechanism underlying the function of circZFR. Functionally, circZFR knockdown represses the proliferation, invasion, and tumor growth. Furthermore, circRNA pull-down experiments combined with mass spectrometry unveil the interactions of circZFR with Single-Stranded DNA Binding Protein 1 (SSBP1). Mechanistically, circZFR bound with SSBP1, thereby promoting the assembly of CDK2/cyclin E1 complexes. The activation of CDK2/cyclin E1 complexes induced p-Rb phosphorylation, thus releasing activated E2F1 leading to cell cycle progression and cell proliferation. 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subjects Antibodies
Biomarkers
Cell cycle
Cell cycle regulation
Cell growth
Cervical cancer
Chemotherapy
Circular RNAs
Development and progression
Gene expression
Mass spectrometry
MicroRNAs
Phosphorylation
Protein binding
Proteins
Radiation therapy
RNA
RNA binding protein
RNA Immunoprecipitation
Software
Transcription factors
Tumors
Wound healing
title The circular RNA circZFR phosphorylates Rb promoting cervical cancer progression by regulating the SSBP1/CDK2/cyclin E1 complex
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