In vitro study of nanoliposomes containing curcumin and doxycycline for enhanced antimicrobial photodynamic therapy against Aggregatibacter actinomycetemcomitans

The excessive inappropriate use of systemic antibiotics has contributed to the emergence of antibiotic-resistant pathogens, which pose a significant risk to the success of treatment. This study has approached this problem by developing doxycycline-loaded liposome doped with curcumin (NL-Cur +Dox ) f...

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Bibliographic Details
Published in:Scientific reports 2023-07, Vol.13 (1), p.11552-11552, Article 11552
Main Authors: Afrasiabi, Shima, Partoazar, Alireza, Chiniforush, Nasim
Format: Article
Language:English
Subjects:
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Aggregatibacter actinomycetemcomitans
Anti-Bacterial Agents - pharmacology
Antibiotic resistance
Antibiotics
Biofilms
Controlled release
Crystallization
Curcumin
Curcumin - chemistry
Cytotoxicity
Doxycycline
Doxycycline - pharmacology
Humanities and Social Sciences
Humans
Metabolism
multidisciplinary
Periodontitis
Photochemotherapy
Photodynamic therapy
Science
Science (multidisciplinary)
Transmission electron microscopy
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Summary:The excessive inappropriate use of systemic antibiotics has contributed to the emergence of antibiotic-resistant pathogens, which pose a significant risk to the success of treatment. This study has approached this problem by developing doxycycline-loaded liposome doped with curcumin (NL-Cur +Dox ) for combination antibacterial therapy against Aggregatibacter actinomycetemcomitans . The characterization of formulation revealed encapsulation of both drugs in NL-Cur +Dox with an average size of 239 nm and sustained release behavior. Transmission electron microscopy analysis confirmed the vesicular-shaped nanocarriers without any aggregation or crystallization. The cytotoxic and hemolytic activities of NL-Cur +Dox were evaluated. The anti-biofilm and anti-metabolic effects of NL-Cur +Dox -mediated antimicrobial photodynamic therapy (aPDT) were examined. The data indicated that NL-Cur +Dox -mediated aPDT led to a significant reduction of biofilm (82.7%, p  = 0.003) and metabolic activity (75%, p  
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-023-38812-4