In vitro study of nanoliposomes containing curcumin and doxycycline for enhanced antimicrobial photodynamic therapy against Aggregatibacter actinomycetemcomitans

The excessive inappropriate use of systemic antibiotics has contributed to the emergence of antibiotic-resistant pathogens, which pose a significant risk to the success of treatment. This study has approached this problem by developing doxycycline-loaded liposome doped with curcumin (NL-Cur +Dox ) f...

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Published in:Scientific reports 2023-07, Vol.13 (1), p.11552-11552, Article 11552
Main Authors: Afrasiabi, Shima, Partoazar, Alireza, Chiniforush, Nasim
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Language:English
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631/326
639/925
692/308
692/699
692/700
Aggregatibacter actinomycetemcomitans
Anti-Bacterial Agents - pharmacology
Antibiotic resistance
Antibiotics
Biofilms
Controlled release
Crystallization
Curcumin
Curcumin - chemistry
Cytotoxicity
Doxycycline
Doxycycline - pharmacology
Humanities and Social Sciences
Humans
Metabolism
multidisciplinary
Periodontitis
Photochemotherapy
Photodynamic therapy
Science
Science (multidisciplinary)
Transmission electron microscopy
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description The excessive inappropriate use of systemic antibiotics has contributed to the emergence of antibiotic-resistant pathogens, which pose a significant risk to the success of treatment. This study has approached this problem by developing doxycycline-loaded liposome doped with curcumin (NL-Cur +Dox ) for combination antibacterial therapy against Aggregatibacter actinomycetemcomitans . The characterization of formulation revealed encapsulation of both drugs in NL-Cur +Dox with an average size of 239 nm and sustained release behavior. Transmission electron microscopy analysis confirmed the vesicular-shaped nanocarriers without any aggregation or crystallization. The cytotoxic and hemolytic activities of NL-Cur +Dox were evaluated. The anti-biofilm and anti-metabolic effects of NL-Cur +Dox -mediated antimicrobial photodynamic therapy (aPDT) were examined. The data indicated that NL-Cur +Dox -mediated aPDT led to a significant reduction of biofilm (82.7%, p  = 0.003) and metabolic activity (75%, p  
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subjects 631/326
639/925
692/308
692/699
692/700
Aggregatibacter actinomycetemcomitans
Anti-Bacterial Agents - pharmacology
Antibiotic resistance
Antibiotics
Biofilms
Controlled release
Crystallization
Curcumin
Curcumin - chemistry
Cytotoxicity
Doxycycline
Doxycycline - pharmacology
Humanities and Social Sciences
Humans
Metabolism
multidisciplinary
Periodontitis
Photochemotherapy
Photodynamic therapy
Science
Science (multidisciplinary)
Transmission electron microscopy
title In vitro study of nanoliposomes containing curcumin and doxycycline for enhanced antimicrobial photodynamic therapy against Aggregatibacter actinomycetemcomitans
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