Chemical Fingerprinting Profile and Targeted Quantitative Analysis of Phenolic Compounds from Rooibos Tea (Aspalathus linearis) and Dietary Supplements Using UHPLC-PDA-MS

Aspalathus linearis (Burm.f.) R. Dahlgren, commonly known as rooibos tea, was consumed traditionally by the indigenous South African inhabitants as an herbal remedy. Beside antioxidant properties, it displays antiallergic, antispasmodic, and hypoglycemic activities. An ultra-high-performance liquid...

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Bibliographic Details
Published in:Separations 2022-07, Vol.9 (7), p.159
Main Authors: Fantoukh, Omer I., Wang, Yan-Hong, Parveen, Abidah, Hawwal, Mohammed F., Ali, Zulfiqar, Al-Hamoud, Gadah A., Chittiboyina, Amar G., Joubert, Elizabeth, Viljoen, Alvaro, Khan, Ikhlas A.
Format: Article
Language:English
Subjects:
Acetonitrile
Antioxidants
aspalathin
Aspalathus linearis
Chemical fingerprinting
Cost analysis
dietary flavonoids
Dietary supplements
Flavonoids
Formic acid
High performance liquid chromatography
Leguminosae
Mass spectrometry
Metabolites
nothofagin
Phenols
Photodiodes
Phytochemicals
Quality control
Quality standards
Quantitative analysis
rooibos
Scientific imaging
Separation
Tea
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Summary:Aspalathus linearis (Burm.f.) R. Dahlgren, commonly known as rooibos tea, was consumed traditionally by the indigenous South African inhabitants as an herbal remedy. Beside antioxidant properties, it displays antiallergic, antispasmodic, and hypoglycemic activities. An ultra-high-performance liquid chromatography method coupled with photodiode array and mass spectrometry detectors were developed for the determination of 14 phenolic constituents from leaves and stems of A. linearis. The efficient separation was performed within 30 min at a temperature of 30 °C by using C-18 column as the stationary phase and water/acetonitrile with 0.05% formic acid as the mobile phase. Method validation for linearity, repeatability, limits of detection, and limits of quantification was achieved. The limits of detection from 0.2–1 μg/mL were reported for the standard compounds. Their total content varied substantially (1.50–9.85 mg/100 mg sample) in 21 dietary supplements. The presence of regioisomers and diastereomers which co-elute on a variety of stationary phases make separation for quantification purposes challenging. This method was found to be efficient in providing low retention times and excellent resolution for this type of phytochemicals. The established method is suitable for chemical fingerprint analysis of A. linearis and cost-effective for quality control of rooibos tea products.
ISSN:2297-8739
2297-8739
DOI:10.3390/separations9070159