Cellular entry and uncoating of naked and quasi-enveloped human hepatoviruses

Many 'non-enveloped' viruses, including hepatitis A virus (HAV), are released non-lytically from infected cells as infectious, quasi-enveloped virions cloaked in host membranes. Quasi-enveloped HAV (eHAV) mediates stealthy cell-to-cell spread within the liver, whereas stable naked virions...

Full description

Saved in:
Bibliographic Details
Published in:eLife 2019-02, Vol.8
Main Authors: Rivera-Serrano, Efraín E, González-López, Olga, Das, Anshuman, Lemon, Stanley M
Format: Article
Language:English
Subjects:
Biosynthesis
Cell Line
Clathrin
Cytosol
Dynamin
endocytic trafficking
Endocytosis
Endosomes
exosomes
extracellular vesicles
Genomes
Heparan sulfate
Hepatitis
Hepatitis A
Hepatitis A virus - physiology
Hepatocytes
Hepatocytes - virology
Humans
Infections
integrins
Lipids
Lysosomes
Lysosomes - virology
Medical research
Microbiology and Infectious Disease
Phospholipase
picornavirus
PLA2G16
Protein transport
Proteins
Uncoating
Virions
Virus Internalization
Virus Uncoating
Viruses
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Many 'non-enveloped' viruses, including hepatitis A virus (HAV), are released non-lytically from infected cells as infectious, quasi-enveloped virions cloaked in host membranes. Quasi-enveloped HAV (eHAV) mediates stealthy cell-to-cell spread within the liver, whereas stable naked virions shed in feces are optimized for environmental transmission. eHAV lacks virus-encoded surface proteins, and how it enters cells is unknown. We show both virion types enter by clathrin- and dynamin-dependent endocytosis, facilitated by integrin β , and traffic through early and late endosomes. Uncoating of naked virions occurs in late endosomes, whereas eHAV undergoes ALIX-dependent trafficking to lysosomes where the quasi-envelope is enzymatically degraded and uncoating ensues coincident with breaching of endolysosomal membranes. Neither virion requires PLA2G16, a phospholipase essential for entry of other picornaviruses. Thus naked and quasi-enveloped virions enter via similar endocytic pathways, but uncoat in different compartments and release their genomes to the cytosol in a manner mechanistically distinct from other .
ISSN:2050-084X
2050-084X
DOI:10.7554/elife.43983