Effects of Feeding 60% Dried Corn Distillers’ Grains or the Equivalent Sulfur as CaSO4 on DNA Integrity and Gene Expression in Yearling Angus Bull Sperm

• Published in: Ruminants 2023-12, Vol.3 (4), p.286-298
• Main Authors: Kassetas, Cierrah J., Geary, Tom W., Zezeski, Abby L., Caton, Joel S., Kirsch, James D., Dorsam, Sheri T., Diniz, Wellison J. S., McCarthy, Kacie L., Crouse, Matthew S., Sedivec, Kevin K., Neville, Bryan W., Dahlen, Carl R.
• Format: Article
• Language: English
• Subjects: beef bulls; distillers’ grains plus solubles; DNA integrity; gene sequencing; sperm
• ISSN: 2673-933X; 2673-933X
• DOI: 10.3390/ruminants3040026

We evaluated the effects of feeding 60% dried corn distillers’ grains plus solubles (DDGS) or the equivalent sulfur as CaSO4 on sperm characteristics and transcript abundance. Thirty-six half-sibling Angus bulls (256 ± 8.5 d; initial BW = 320 ± 2.7 kg) were assigned to one of three treatments: (1) 60% concentrate as corn (CON); (2) 60% DDGS as corn replacement (60DDGS); and (3) CON diet + equivalent sulfur of 60DDGS added as CaSO4 (SULF). The acrosome/cell membrane integrity, mitochondrial energy potential, oxidation status, DNA integrity, and zinc signatures were analyzed via flow cytometry. Sperm-specific gene expression was assessed via RNA sequencing. The flow cytometry data were analyzed using PROC MIXED in SAS to determine the effects of treatment. Pairwise comparisons based on edgeR were used to identify differentially expressed genes. The percentage of polarized mitochondria tended to be greater (p = 0.08) for SULF compared with CON and 60DDGS. Protamine 1 was upregulated (p < 0.01; FDR = 0.10) in 60DDGS compared to CON. Zinc signature 1 in 60DDGS and SULF was reduced (p = 0.03) compared to CON. This study suggests that feeding bulls diets containing 60% DDGS had little effect on DNA integrity and gene expression.