Expression and Function of ABC Transporters in Human Alveolar Epithelial Cells

ATP-binding cassette (ABC) transporters are a large superfamily of membrane transporters that facilitate the translocation of different substrates. While ABC transporters are clearly expressed in various tumor cells where they can play a role in drug extrusion, the presence of these transporters in...

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Bibliographic Details
Published in:Biomolecules (Basel, Switzerland) Switzerland), 2022-09, Vol.12 (9), p.1260
Main Authors: Visigalli, Rossana, Rotoli, Bianca Maria, Ferrari, Francesca, Di Lascia, Maria, Riccardi, Benedetta, Puccini, Paola, Dall’Asta, Valeria, Barilli, Amelia
Format: Article
Language:English
Subjects:
ABC transporters
alveolar epithelium
Alveoli
ATP-binding cassette transporters
BCRP
Carrier proteins
Cell culture
Epithelial cells
Epithelium
Fibroblasts
Gene expression
Genetic aspects
Health aspects
Homology
MDR1
MDR1 protein
MRP1
Permeability
Physiology
Proteins
Pulmonary alveoli
Tumor cells
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Summary:ATP-binding cassette (ABC) transporters are a large superfamily of membrane transporters that facilitate the translocation of different substrates. While ABC transporters are clearly expressed in various tumor cells where they can play a role in drug extrusion, the presence of these transporters in normal lung tissues is still controversial. Here, we performed an analysis of ABC transporters in EpiAlveolarTM, a recently developed model of human alveoli, by defining the expression and activity of MDR1, BCRP, and MRPs. Immortalized primary epithelial cells hAELVi (human alveolar epithelial lentivirus-immortalized cells) were employed for comparison. Our data underline a close homology between these two models, where none of the ABC transporters here studied are expressed on the apical membrane and only MRP1 is clearly detectable and functional at the basolateral side. According to these findings, we can conclude that other thus-far-unidentified transporter/s involved in drug efflux from alveolar epithelium deserve investigations.
ISSN:2218-273X
2218-273X
DOI:10.3390/biom12091260