Dissemination of Neisseria gonorrhoeae with decreased susceptibility to extended-spectrum cephalosporins in Southern China, 2021: a genome-wide surveillance from 20 cities

• Published in: Annals of clinical microbiology and antimicrobials 2023-05, Vol.22 (1), p.39-39, Article 39
• Main Authors: Liao, Yiwen, Xie, Qinghui, Li, Xiaoxiao, Yin, Xiaona, Wu, Xingzhong, Liu, Mingjing, Pan, Yuying, Zeng, Lihong, Yang, Jianjiang, Feng, Zhanqin, Qin, Xiaolin, Zheng, Heping
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - pharmacology; Anti-Bacterial Agents - therapeutic use; Antibiotics; Antimicrobial agents; Antimicrobial resistance; Azithromycin; Cefixime; Cefixime - pharmacology; Ceftriaxone; Ceftriaxone - pharmacology; Cephaloridine; Cephalosporins; Cephalosporins - pharmacology; Ciprofloxacin; Cities; Cloning; Control; Dosage and administration; Drug resistance in microorganisms; Epidemics; Extended-spectrum cephalosporins (ESCs); Gene sequencing; Genes; Genomes; Genomic epidemiology; Genotyping; Gonorrhea; Gonorrhea - drug therapy; Gonorrhea - epidemiology; Humans; Identification and classification; Microbial Sensitivity Tests; Minimum inhibitory concentration; Moxalactam; Multilocus Sequence Typing; Mutation; Neisseria gonorrhoeae; Neisseria gonorrhoeae - genetics; Nucleotide sequence; Penicillin; Phylogenetics; Phylogeny; Prevention; Resistance; Sexually transmitted diseases; Sexually transmitted infections; Software; Spectinomycin; Surveillance; Susceptibility; Whole genome sequencing
• ISSN: 1476-0711; 1476-0711
• DOI: 10.1186/s12941-023-00587-x

Antimicrobial resistance (AMR) of untreatable gonococcal infection is an emerging threat, especially in Guangdong, a prosperous province in Southern China. N.gonorrhoeae was isolated from 20 cities in Guangdong and determined antimicrobial susceptibility. Through whole-genome sequencing (WGS), multilocus sequence typing (MLST), N.gonorrhoeae multiantigen sequence typing (NG-MAST), and N.gonorrhoeae sequence typing for antimicrobial resistance (NG-STAR) were obtained based on the PubMLST database ( https://pubmlst.org/ ). Phylogenetic analysis was used for dissemination and tracking analysis. Antimicrobial susceptibility was performed on 347 isolates, and 50 isolates were identified as decreased susceptibility (DS) to cephalosporins. Of which 16.0% (8/50) were ceftriaxone DS, 38.0% (19/50) were cefixime DS, and 46.0% (23/50) were both ceftriaxone and cefixime DS. In all, the dual-resistant rate of the cephalosporin-DS isolates was 96.0% for penicillin and 98.0% for tetracycline-resistant, and 10.0% (5/50) were resistant to azithromycin. All cephalosporin-DS isolates were resistant to ciprofloxacin but sensitive to spectinomycin. The predominant MLSTs were ST7363 (16%, 8/50), ST1903 (14%, 7/50), ST1901 (12%, 6/50), and ST7365 (10%, 5/50). Besides some isolates that failed genotyping (NA), NG-STAR ST1143 (n = 6) and NG-MAST ST17748 (n = 4) were the most prevalent. Twelve isolates with mosaic penA-60.001 allele retained the most elevated cephalosporin MIC (Minimum Inhibitory Concentration). Phylogenetic analysis revealed that epidemic penA-60.001 clones, either domestic or foreign, had spread to nine cities in Guangdong, and 9/12 clones were from the Pearl River Delta region. N. gonorrhoeae with cephalosporins-DS was extensively disseminated in Guangdong, Southern China, requiring strict surveillance.