Development of a microwave-based extraction for forensic biological samples

In this study, a quick microwave-based treatment was developed as a front end for DNA analysis of forensic samples. The effect of microwave treatment is to cause cell disruption which can improve the release of DNA during direct PCR as well as with extraction methods. Exposure to microwave preproces...

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Bibliographic Details
Published in:Forensic science international. Synergy 2022-01, Vol.5, p.100291, Article 100291
Main Authors: Taglia, Fabiana, Wang, Ling, Setser, Casandra H., Fernández-Tejero, Nicole, McCord, Bruce R., Lee, Steven B.
Format: Article
Language:English
Subjects:
Direct PCR
Forensic analysis
Interdisciplinary Forensics
Microwave
Microwave extraction
Rapid DNA
Saliva
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Summary:In this study, a quick microwave-based treatment was developed as a front end for DNA analysis of forensic samples. The effect of microwave treatment is to cause cell disruption which can improve the release of DNA during direct PCR as well as with extraction methods. Exposure to microwave preprocessing improved the quality of rapid genotyping, particularly when used with low level samples. Optimal results were obtained when samples were microwaved at 300W for 40 s, resulting in improved allele detection. Overall, the addition of this simple preprocessing step improves sensitivity and allele recovery for low level DNA samples when combined with expedited DNA analysis workflows. Its main advantages include speed, low cost, compatibility with downstream DNA methods and application to a wide variety of samples.
ISSN:2589-871X
2589-871X
DOI:10.1016/j.fsisyn.2022.100291