Effect of Bioactive Ingredients on Urinary Excretion of Aflatoxin B1 and Ochratoxin A in Rats, as Measured by Liquid Chromatography with Fluorescence Detection

Aflatoxin B1 (AFB1) and ochratoxin A (OTA) are highly toxic mycotoxins present in food and feed, posing serious health risks to humans and animals. This study aimed to validate an efficient and cost-effective analytical method for quantifying AFB1 and OTA in rat urine using immunoaffinity column ext...

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Bibliographic Details
Published in:Toxins 2024-08, Vol.16 (8), p.363
Main Authors: Vila-Donat, Pilar, Sánchez, Dora, Cimbalo, Alessandra, Mañes, Jordi, Manyes, Lara
Format: Article
Language:English
Subjects:
Aflatoxin B1
Aflatoxin B1 - urine
Aflatoxins
Animal Feed - analysis
Animals
Biocompatibility
Biomarkers
Carcinogens
Chromatography
Chromatography, Liquid
Column chromatography
Cost analysis
Costs (Law)
Excretion
feed
Feeds
Female
Fluorescence
Food contamination & poisoning
Food Contamination - analysis
Food products
functional compounds
Health aspects
Health risks
In vivo methods and tests
Instrument industry
International economic relations
Liquid chromatography
Male
Metabolism
Metabolites
Methods
mycotoxin excretion
Mycotoxins
Ochratoxin A
Ochratoxins - urine
Pumpkins
Rats
Rats, Sprague-Dawley
Spectrometry, Fluorescence
Urine
Whey
Whey - chemistry
Wistar rats
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Summary:Aflatoxin B1 (AFB1) and ochratoxin A (OTA) are highly toxic mycotoxins present in food and feed, posing serious health risks to humans and animals. This study aimed to validate an efficient and cost-effective analytical method for quantifying AFB1 and OTA in rat urine using immunoaffinity column extraction and liquid chromatography with fluorescence detection (IAC-LC-FD). Additionally, the study evaluated the effect of incorporating fermented whey and pumpkin into the feed on the urinary excretion of these mycotoxins. The limits of detection and quantification were determined to be 0.1 µg/kg and 0.3 µg/kg, respectively, for both mycotoxins in feed, and 0.2 ng/mL and 0.6 ng/mL, respectively, in urine. The method demonstrated robust recovery rates ranging from 74% to 119% for both AFB1 and OTA in both matrices. In feed samples, the levels of AFB1 and OTA ranged from 4.3 to 5.2 µg/g and from 5.4 to 8.8 µg/g, respectively. This validated method was successfully applied to analyze 116 urine samples from rats collected during the fourth week of an in vivo trial. The results indicated that the addition of fermented whey and pumpkin to the feed influenced mycotoxin excretion in urine, with variations observed based on the sex of the rats, type of mycotoxin, and exposure dosage.
ISSN:2072-6651
2072-6651
DOI:10.3390/toxins16080363