In Silico Designed Multi-Epitope Immunogen “Tpme-VAC/LGCM-2022” May Induce Both Cellular and Humoral Immunity against Treponema pallidum Infection

Syphilis, a sexually transmitted infection caused by the spirochete Treponema pallidum, has seen a resurgence over the past years. T. pallidum is capable of early dissemination and immune evasion, and the disease continues to be a global healthcare burden. The purpose of this study was to design a m...

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Published in:Vaccines (Basel) 2022-06, Vol.10 (7), p.1019
Main Authors: Gomes, Lucas Gabriel Rodrigues, Rodrigues, Thaís Cristina Vilela, Jaiswal, Arun Kumar, Santos, Roselane Gonçalves, Kato, Rodrigo Bentes, Barh, Debmalya, Alzahrani, Khalid J., Banjer, Hamsa Jameel, Soares, Siomar de Castro, Azevedo, Vasco, Tiwari, Sandeep
Format: Article
Language:English
Subjects:
Allergenicity
Antigenicity
chimeric multi-epitope vaccine
Cloning
Cloning vectors
Congenital diseases
Dynamic stability
E coli
Epitopes
Genomes
Global health
HIV
Human immunodeficiency virus
Humoral immunity
Immune response
Immune system
immunoinformatics
Infections
Molecular docking
Pathogens
Penicillin
Population
Proteins
Sex industry
Sexually transmitted diseases
sexually transmitted infection
Spirochetes
STD
Syphilis
TLR2 protein
Toll-like receptors
Treponema pallidum
Vaccines
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Summary:Syphilis, a sexually transmitted infection caused by the spirochete Treponema pallidum, has seen a resurgence over the past years. T. pallidum is capable of early dissemination and immune evasion, and the disease continues to be a global healthcare burden. The purpose of this study was to design a multi-epitope immunogen through an immunoinformatics-based approach. Multi-epitope immunogens constitute carefully selected epitopes belonging to conserved and essential bacterial proteins. Several physico-chemical characteristics, such as antigenicity, allergenicity, and stability, were determined. Further, molecular docking and dynamics simulations were performed, ensuring binding affinity and stability between the immunogen and TLR-2. An in silico cloning was performed using the pET-28a(+) vector and codon adaptation for E. coli. Finally, an in silico immune simulation was performed. The in silico predictions obtained in this work indicate that this construct would be capable of inducing the requisite immune response to elicit protection against T. pallidum. Through this methodology we have designed a promising potential vaccine candidate for syphilis, namely Tpme-VAC/LGCM-2022. However, it is necessary to validate these findings in in vitro and in vivo assays.
ISSN:2076-393X
2076-393X
DOI:10.3390/vaccines10071019