Generation and characterization of two iPSC lines from human adipose tissue-derived stem cells of healthy donors

•hASCs can be easily and less invasively obtained from adipose tissues clinically.•iPSCs can be reprogrammed from hASCs via nonintegrative Sendai viral vector system.•The iPSCs derived cells can serve as control for disease modeling and drug screening. Here we have generated two induced pluripotent...

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Published in:Stem cell research 2020-10, Vol.48, p.101973, Article 101973
Main Authors: Zhou, Xiang-Bin, Li, Hong, Li, Fei, Song, Xian-Kui, Liu, Tun, Ma, Tian, Guo, Hong-Yan, Wu, Ning, Li, Jin
Format: Article
Language:English
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Summary:•hASCs can be easily and less invasively obtained from adipose tissues clinically.•iPSCs can be reprogrammed from hASCs via nonintegrative Sendai viral vector system.•The iPSCs derived cells can serve as control for disease modeling and drug screening. Here we have generated two induced pluripotent stem cell (iPSC) lines, hASC-iPSC-1A and hASC-iPSC-2A, by reprogramming human adipose tissue derived stem cells of 28 and 23 years old healthy donors. Reprogramming was achieved using nonintegrative Sendai viral vector system containing the reprogramming factors Klf4, Oct3/4, Sox2, c-Myc. Though the karyotypes of these cells were normal (46, XX) and (46, XY), their pluripotency potentials were confirmed by the expression of factors of pluripotency markers in vitro and teratoma formation in vivo. These iPSCs differentiated cells can serve as control for disease modeling and drug screening.
ISSN:1873-5061
1876-7753
DOI:10.1016/j.scr.2020.101973