Hepatocellular carcinoma patients serum modulates the regenerative capacities of adipose mesenchymal stromal cells

Hepatocellular carcinoma (HCC) is one of the most prevalent cancers causing the highest mortality rate worldwide. Treatment options of surgery, radiation, cytotoxic drugs and liver transplantation suffer significant side effects and a high frequency of relapse. Stem cell therapy has been proposed as...

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Published in:Heliyon 2024-02, Vol.10 (3), p.e24794-e24794, Article e24794
Main Authors: Salah, Radwa Ayman, El-Derby, Azza M., El-Gammal, Zaynab, Wadie, Bishoy, Ahmed, Sara M., Elshenawy, Shimaa E., Magdy, Shireen, Salah, Ayman, Gabr, Mahmoud, Mohamed, Ihab, El-Badri, Nagwa
Format: Article
Language:English
Subjects:
Adipose mesenchymal stem cells
HCC serum
Hepatic cancer cell line
huh7 cells
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Summary:Hepatocellular carcinoma (HCC) is one of the most prevalent cancers causing the highest mortality rate worldwide. Treatment options of surgery, radiation, cytotoxic drugs and liver transplantation suffer significant side effects and a high frequency of relapse. Stem cell therapy has been proposed as a new effective therapy, however, controversial reports are emerging on the role of mesenchymal stem cells in cancer. In this work, we aimed to assess the regenerative capacities of adipose mesenchymal stem cells when exposed to serum from HCC patients, by assessing the effect of the sera on modulating the regenerative capacities of h-AMSCs and the cancer properties in HCC cells. This will pave the way for maximizing the efficacy of MSCs in cancer therapy. Our data show that HCC serum-treated hA-MSCs suffered oncogene-induced senescence as shown by their altered morphology and ameliorated proliferation and differentiation. The cells were enlarged with small irregular nuclei, swollen rough endoplasmic reticulum cisternae, and aging lysosomes typified by dark residual bodies. HCC serum-treated Huh-7 cancer cells on the other hand displayed higher tumor aggressiveness as depicted by altered morphology, increased cellular proliferation and migration, and decreased percentage of early and late apoptotic cells. Our findings provide evidence that exposure of hA-MSCs to the serum of HCC patients decreases their regenerative capacities and should be considered when employed as a potential therapy in HCC patients.
ISSN:2405-8440
2405-8440
DOI:10.1016/j.heliyon.2024.e24794