Genetic organization of an M protein trans-acting positive regulator (Mga) orthologue and its adjacent M-like protein (SCM) alleles in Streptococcus canis

• Published in: BMC research notes 2024-05, Vol.17 (1), p.138-9, Article 138
• Main Authors: Takahashi, Takashi, Maeda, Takahiro, Yoshida, Haruno, Goto, Mieko, Tsuyuki, Yuzo, Kim, Jae-Seok
• Format: Article
• Language: English
• Subjects: Alleles; Amino Acid Sequence; Animals; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Genes; Genetic organization; M protein; M protein trans-acting positive regulator (Mga); M-like protein; M-like protein (SCM); Pathogens; Peptides; Phylogenetics; Population genetics; Proteins; Research Note; Streptococcus - genetics; Streptococcus canis; Streptococcus infections
• ISSN: 1756-0500; 1756-0500
• DOI: 10.1186/s13104-024-06795-8

The purpose of this study was to identify the M protein trans-acting positive regulator (Mga) orthologue and its adjacent M-like protein (SCM) alleles in Streptococcus canis. Using the 39 SCM allele isolates and polymerase chain reaction-based amplification and sequencing, we obtained the deduced Mga amino acid (AA) sequences. The 22 Mga sequences in whole-genome sequences were obtained by searching the National Collection of Type Cultures 12,191(T) Mga sequence into the database. The percentage identity to the type-strain Mga sequence was examined along with its size. The presence of the Mga-specific motifs was confirmed. Of the 62 strains, we identified 59 Mga sequences with an AA size of 509 (except for four different sizes). Percentage identity ranged from 96.66 to 100% with the confirmed Mga-specific motifs and diverse SCM allele populations. Our findings support the presence of an Mga orthologue and diverse SCM allele populations.