Investigation of antibacterial properties silver nanoparticles prepared via green method

• Published in: BMC chemistry 2012-07, Vol.6 (1), p.73-73, Article 73
• Main Authors: Shameli, Kamyar, Ahmad, Mansor Bin, Jazayeri, Seyed Davoud, Shabanzadeh, Parvaneh, Sangpour, Parvanh, Jahangirian, Hossein, Gharayebi, Yadollah
• Format: Article
• Language: English
• Subjects: Antibacterial activity; Antiinfectives and antibacterials; Aqueous solutions; Bacteria; Biocompatibility; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Fourier transforms; Green chemistry; HIV; Human immunodeficiency virus; Inhibitors; Materials and Polymers; Microbiology; Microscopy; Nanocrystals; Nanoparticles; Polyethylene glycol; Polymer blends; Reaction time effect; Research Article; Salmonella typhimurium; Silver; Silver nanoparticles; Stirring; Temperature; X-ray diffraction
• ISSN: 1752-153X; 1752-153X; 2661-801X
• DOI: 10.1186/1752-153X-6-73

Background This study aims to investigate the influence of different stirring times on antibacterial activity of silver nanoparticles in polyethylene glycol (PEG) suspension. The silver nanoparticles (Ag-NPs) were prepared by green synthesis method using green agents, polyethylene glycol (PEG) under moderate temperature at different stirring times. Silver nitrate (AgNO 3 ) was taken as the metal precursor while PEG was used as the solid support and polymeric stabilizer. The antibacterial activity of different sizes of nanosilver was investigated against Gram–positive [ Staphylococcus aureus ] and Gram–negative bacteria [ Salmonella typhimurium SL1344 ] by the disk diffusion method using Müeller–Hinton Agar. Results Formation of Ag-NPs was determined by UV–vis spectroscopy where surface plasmon absorption maxima can be observed at 412–437 nm from the UV–vis spectrum. The synthesized nanoparticles were also characterized by X-ray diffraction (XRD). The peaks in the XRD pattern confirmed that the Ag-NPs possessed a face-centered cubic and peaks of contaminated crystalline phases were unable to be located. Transmission electron microscopy (TEM) revealed that Ag-NPs synthesized were in spherical shape. The optimum stirring time to synthesize smallest particle size was 6 hours with mean diameter of 11.23 nm. Zeta potential results indicate that the stability of the Ag-NPs is increases at the 6 h stirring time of reaction. The Fourier transform infrared (FT-IR) spectrum suggested the complexation present between PEG and Ag-NPs. The Ag-NPs in PEG were effective against all bacteria tested. Higher antibacterial activity was observed for Ag-NPs with smaller size. These suggest that Ag-NPs can be employed as an effective bacteria inhibitor and can be applied in medical field. Conclusions Ag-NPs were successfully synthesized in PEG suspension under moderate temperature at different stirring times. The study clearly showed that the Ag-NPs with different stirring times exhibit inhibition towards the tested gram-positive and gram-negative bacteria.