Best Practices for Comprehensive Annotation of Neuropeptides of Gryllus bimaculatus

Genome annotation is critically important data that can support research. Draft genome annotations cover representative genes; however, they often do not include genes that are expressed only in limited tissues and stages, or genes with low expression levels. Neuropeptides are responsible for regula...

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Bibliographic Details
Published in:Insects (Basel, Switzerland) Switzerland), 2023-01, Vol.14 (2), p.121
Main Authors: Mochizuki, Takako, Sakamoto, Mika, Tanizawa, Yasuhiro, Seike, Hitomi, Zhu, Zhen, Zhou, Yi Jun, Fukumura, Keisuke, Nagata, Shinji, Nakamura, Yasukazu
Format: Article
Language:English
Subjects:
Amino acids
Annotations
Assembly
Best practice
Biological activity
Crickets
Datasets
draft genome annotation
functional annotation
Gene expression
Gene loci
Gene mapping
Gene sequencing
Genes
Genetic engineering
genome annotation
Genomes
Genomics
Gryllus bimaculatus
Insects
Neuropeptides
Next-generation sequencing
Peptides
Physiology
Proteins
Transcriptomes
Transcriptomics
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Summary:Genome annotation is critically important data that can support research. Draft genome annotations cover representative genes; however, they often do not include genes that are expressed only in limited tissues and stages, or genes with low expression levels. Neuropeptides are responsible for regulation of various physiological and biological processes. A recent study disclosed the genome draft of the two-spotted cricket , which was utilized to understand the intriguing physiology and biology of crickets. Thus far, only two of the nine reported neuropeptides in were annotated in the draft genome. Even though de novo assembly using transcriptomic analyses can comprehensively identify neuropeptides, this method does not follow those annotations on the genome locus. In this study, we performed the annotations based on the reference mapping, de novo transcriptome assembly, and manual curation. Consequently, we identified 41 neuropeptides out of 43 neuropeptides, which were reported in the insects. Further, 32 of the identified neuropeptides on the genomic loci in were annotated. The present annotation methods can be applicable for the neuropeptide annotation of other insects. Furthermore, the methods will help to generate useful infrastructures for studies relevant to neuropeptides.
ISSN:2075-4450
2075-4450
DOI:10.3390/insects14020121