Genetic study in patients operated dentally and anesthetized with articaine-epinephrine

In this study we wanted to figure out if there was a correlation between OPRM1 N40D, TRPV1 I316M, TRPV1 I585V, NOS3 -786T>C and IL6 -174C>G polymorphisms and the response to locally applied articaine-epinephrine anesthetic. In this observational study, 114 oral cell samples of patients anesthe...

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Bibliographic Details
Published in:Journal of pain research 2019-04, Vol.12, p.1371-1384
Main Authors: López-Valverde, Nansi, López-Valverde, Antonio, Gómez de Diego, Rafael, Cieza-Borrella, Clara, Ramírez, Juan M, González-Sarmiento, Rogelio
Format: Article
Language:English
Subjects:
Adrenergic agonists
Analysis
Anesthesia
articaine-epinefrine anesthetic
Central nervous system agents
DNA
Epinephrine
Genetic aspects
Genetic research
IL6
Medical research
NOS3
OPRM1
Original Research
Pain
polymorphism
Time
TRPV1
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Summary:In this study we wanted to figure out if there was a correlation between OPRM1 N40D, TRPV1 I316M, TRPV1 I585V, NOS3 -786T>C and IL6 -174C>G polymorphisms and the response to locally applied articaine-epinephrine anesthetic. In this observational study, 114 oral cell samples of patients anesthetized with articaine-epinephrine (54 from men 60 from women), were collected from dental centers in Madrid (Spain). High molecular weight DNA was obtained from oral mucosa cells. The analysis of OPRM1 N40D (rs1799971), TRPV1 I316M (rs222747), TRPV1 I585V (rs8065080) and IL6 -174C>G polymorphism was performed through real-time PCR allelic discrimination using TaqMan probes. Polymorphism NOS3 -786T> C (rs2070744) was analyzed using RFLP-PCR. The studied polymorphisms are involved neither in the response to the anesthetic, nor in the intensity of perceived dental pain. However, in a subset of female patients we found that TRPV1 I316M was associated with a delayed onset of anesthesia. There is no association among these polymorphisms and the time elapsed between the application of the anesthetic and the onset of its effect.
ISSN:1178-7090
1178-7090
DOI:10.2147/JPR.S193745