Contrasting effects of NADPH oxidases on the fungal hyphae growth and immune responses in Pleurotus ostreatus

• Published in: Frontiers in microbiology 2024-06, Vol.15, p.1387643
• Main Authors: Li, Huiping, Zhu, Jiachun, Li, Zihao, Xu, Ping, Ma, Lin, Zou, Yajie, Qu, Shaoxuan, Wu, Xiaoqin
• Format: Article
• Language: English
• Subjects: fungal defense; H2O2; Microbiology; NADPH oxidases; oyster mushroom; ROS
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2024.1387643

is one of the most consumed mushroom species, as it serves as a high-quality food, favors a rich secondary metabolism, and has remarkable adaptability to the environment and predators. In this study, we investigated the function of two key reactive oxygen species producing enzyme NADPH oxidase (PoNoxA and PoNoxB) in hyphae growth, metabolite production, signaling pathway activation, and immune responses to different stresses. Characterization of the Nox mutants showed that PoNoxB played an important role in the hyphal formation of the multicellular structure, while PoNoxA regulated apical dominance. The ability of to tolerate a series of abiotic stress conditions (e.g., osmotic, oxidative, membrane, and cell-wall stresses) and mechanical damage repair was enhanced with PoNoxA over-expression. PoNoxB had a greater responsibility in regulating the polysaccharide composition of the cell wall and methyl jasmonate and gibberellin GA1 biosynthesis, and improved mushroom resistance against . Moreover, mutants were involved in the jasmonate and GA signaling pathway, and toxic protein defense metabolite production. Our findings shed light on how the oyster mushroom senses stress signals and responds to adverse environments by the complex regulators of Noxs.