Development of a Real-Time PCR Assay for the Detection of the Phlebovirus Fermo Virus

Fermo virus is a Phlebovirus that is increasingly reported in sand flies from northern Italy. The natural cycle is not fully understood, but the virus has been detected by direct methods only in sand flies. Although there is serological evidence that it can infect vertebrates, the virus has not been...

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Bibliographic Details
Published in:Viruses 2023-10, Vol.15 (10), p.2082
Main Authors: Calzolari, Mattia, Russo, Simone, Marzani, Katia, Dalmonte, Gastone, Ricchi, Matteo, Bonilauri, Paolo
Format: Article
Language:English
Subjects:
Biochemical assays
Brief Report
Bunyaviruses
Diagnosis
digital PCR
Efficiency
Epidemiology
Fermo virus
Genetic aspects
Genomes
Identification and classification
Polymerase chain reaction
real-time PCR
RNA polymerase
RNA virus infections
sand fly
Serology
Viruses
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Description
Summary:Fermo virus is a Phlebovirus that is increasingly reported in sand flies from northern Italy. The natural cycle is not fully understood, but the virus has been detected by direct methods only in sand flies. Although there is serological evidence that it can infect vertebrates, the virus has not been directly detected in animals or humans. Here, we have developed and reported a specific real-time PCR for Fermo virus. The availability of the described method will be useful to characterize the epidemiology of the FERV, ensuring, compared to previously available protocols, a more sensitive detection in insects and the possible detection in vertebrates to evaluate the presence of reservoirs and the pathogenic potential of the virus in humans or animals.
ISSN:1999-4915
1999-4915
DOI:10.3390/v15102082