Lack of Conserved miRNA Deregulation in HPV-Induced Squamous Cell Carcinomas

Squamous cell carcinomas (SCCs) in the anogenital and head and neck regions are associated with high-risk types of human papillomaviruses (HR-HPV). Deregulation of miRNA expression is an important contributor to carcinogenesis. This study aimed to pinpoint commonly and uniquely deregulated miRNAs in...

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Bibliographic Details
Published in:Biomolecules (Basel, Switzerland) Switzerland), 2021-05, Vol.11 (5), p.764
Main Authors: Nunvar, Jaroslav, Pagacova, Lucie, Vojtechova, Zuzana, Azevedo, Nayara Trevisan Doimo de, Smahelova, Jana, Salakova, Martina, Tachezy, Ruth
Format: Article
Language:English
Subjects:
Anogenital
Carcinogenesis
Cervical cancer
Etiology
Gene expression
Genital cancers
Genomes
Genomics
Head and neck
Human papillomavirus
Medicine
microRNA
MicroRNAs
miRNA
Mutation
Next-generation sequencing
Papillomaviridae
Principal components analysis
Squamous cell carcinoma
Tonsil
Tumors
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Summary:Squamous cell carcinomas (SCCs) in the anogenital and head and neck regions are associated with high-risk types of human papillomaviruses (HR-HPV). Deregulation of miRNA expression is an important contributor to carcinogenesis. This study aimed to pinpoint commonly and uniquely deregulated miRNAs in cervical, anal, vulvar, and tonsillar tumors of viral or non-viral etiology, searching for a common set of deregulated miRNAs linked to HPV-induced carcinogenesis. RNA was extracted from tumors and nonmalignant tissues from the same locations. The miRNA expression level was determined by next-generation sequencing. Differential expression of miRNAs was calculated, and the patterns of miRNA deregulation were compared between tumors. The total of deregulated miRNAs varied between tumors of different locations by two orders of magnitude, ranging from 1 to 282. The deregulated miRNA pool was largely tumor-specific. In tumors of the same location, a low proportion of miRNAs were exclusively deregulated and no deregulated miRNA was shared by all four types of HPV-positive tumors. The most significant overlap of deregulated miRNAs was found between tumors which differed in location and HPV status (HPV-positive cervical tumors vs. HPV-negative vulvar tumors). Our results imply that HPV infection does not elicit a conserved miRNA deregulation in SCCs.
ISSN:2218-273X
2218-273X
DOI:10.3390/biom11050764