Combined extraction processes of lipid from Chlorella vulgaris microalgae: microwave prior to supercritical carbon dioxide extraction

Extraction yields and fatty acid profiles from freeze-dried Chlorella vulgaris by microwave pretreatment followed by supercritical carbon dioxide (MW-SCCO(2)) extraction were compared with those obtained by supercritical carbon dioxide extraction alone (SCCO(2)). Work performed with pressure range o...

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Bibliographic Details
Published in:International journal of molecular sciences 2011-12, Vol.12 (12), p.9332-9341
Main Authors: Dejoye, Céline, Vian, Maryline Abert, Lumia, Guy, Bouscarle, Christian, Charton, Frederic, Chemat, Farid
Format: Article
Language:English
Subjects:
Algae
Biomass
Carbon dioxide
Carbon Dioxide - chemistry
Cell Fractionation - methods
Chlorella - chemistry
Chlorella - radiation effects
Chlorella vulgaris
Fatty acids
GC/MS
Life Sciences
lipid extraction
Lipids
Lipids - chemistry
Membranes
microalgae
microwave
Microwaves
Photosynthesis
Pressure
Scanning
Solvents
supercritical carbon dioxide
Temperature
Temperature effects
VOCs
Volatile organic compounds
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Summary:Extraction yields and fatty acid profiles from freeze-dried Chlorella vulgaris by microwave pretreatment followed by supercritical carbon dioxide (MW-SCCO(2)) extraction were compared with those obtained by supercritical carbon dioxide extraction alone (SCCO(2)). Work performed with pressure range of 20-28 Mpa and temperature interval of 40-70 °C, gave the highest extraction yield (w/w dry weight) at 28 MPa/40 °C. MW-SCCO(2) allowed to obtain the highest extraction yield (4.73%) compared to SCCO(2) extraction alone (1.81%). Qualitative and quantitative analyses of microalgae oil showed that palmitic, oleic, linoleic and α-linolenic acid were the most abundant identified fatty acids. Oils obtained by MW-SCCO(2) extraction had the highest concentrations of fatty acids compared to SCCO(2) extraction without pretreatment. Native form, and microwave pretreated and untreated microalgae were observed by scanning electronic microscopy (SEM). SEM micrographs of pretreated microalgae present tearing wall agglomerates. After SCCO(2), microwave pretreated microalgae presented several micro cracks; while native form microalgae wall was slightly damaged.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms12129332