Saccharomyces cerevisiae , bentonite, and kaolin as adsorbents for reducing the adverse impacts of mycotoxin contaminated feed on broiler histopathology and hemato-biochemical changes

• Published in: Veterinary World 2021-01, Vol.14 (1), p.23-32
• Main Authors: Ejiofor, Toochukwu, Mgbeahuruike, Anthony Christian, Ojiako, Chiamaka, Ushie, Ashang Micheal, Nwoko, Emmanuela Ifeoma, Onoja, Ibe Remigius, Dada, Toluwase, Mwanza, Mulunda, Karlsson, Magnus
• Format: Article
• Language: English
• Subjects: adsorbent; Aflatoxins; Alanine; Alanine transaminase; Albumin; Alkaline phosphatase; Animal and Dairy Science; Animal feeding and feeds; Aspartate; Aspartate aminotransferase; Bentonite; Birds; Blood; Blood cell count; Bovine spongiform encephalopathy; Bursa of Fabricius; Cell size; Cholesterol; Clay; Contaminants; Deoxynivalenol; Diet; Enzymatic activity; Erythrocytes; feed; Food contamination; Hematology; Hemoglobin; High performance liquid chromatography; Histochemistry; Husdjursvetenskap; Kaolin; Kidneys; Liver; Mold; Molds (Fungi); Mycotoxicosis; mycotoxin; Mycotoxins; pathology; Phosphatases; Saccharomyces cerevisiae; Side effects; Spleen; Transaminase; Yeast
• ISSN: 0972-8988; 2231-0916; 2231-0916
• DOI: 10.14202/vetworld.2021.23-32

, bentonite and kaolin were used to reduce the adverse effects of mold-contaminated diet on broilers. The aim of the study was to evaluate the impact of , bentonite, and kaolin in reducing the adverse effects of mold (fungal) contaminated diet on broilers. Specifically, we investigated the histopathological, hematological, and serum biochemical changes associated with broilers fed mold-contaminated diets supplemented with these three adsorbents. We also isolated and identified the common fungal contaminants in the poultry feeds as well as the mycotoxins they produced. Hundred broilers (3-weeks-old) were randomly grouped into five dietary treatments, basal feed (negative control), feed contaminated with mold, mold-contaminated feed+ , mold-contaminated feed+bentonite, and mold-contaminated feed+kaolin. The fungal contaminants in the feeds were isolated and molecularly identified while the mycotoxins in the feed where analyzed using high-performance liquid chromatography. Blood samples of birds from each group were analyzed for hematology and serum biochemistry. The liver, spleen, kidney, and bursa of Fabricius of the birds were excised and analyzed for histopathological changes. The most common fungal contaminants in the feeds were (33.3%) species, followed by species (22.2%). The mold-contaminated feed had the highest number of fungal contaminants, 55.6%, while the negative control (basal feed group) had none. Total aflatoxin and deoxynivalenol were high in the mold-contaminated feed (53.272 μg/kg and 634.5 μg kg, respectively), but these were reduced by the addition of adsorbents to the feed. The birds fed mold-contaminated feed had significantly (p