Dark septate endophytic fungi associated with pioneer grass inhabiting volcanic deposits and their functions in promoting plant growth

Growth of the pioneer grass Miscanthus condensatus, one of the first vegetation types to be established on volcanic deposits, is promoted by root-associated fungi, particularly dark septate endophytes (DSEs). Fungal taxa within DSEs colonize the root of Miscanthus condensatus in oligotrophic Andosol...

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Bibliographic Details
Published in:Biogeosciences 2023-11, Vol.20 (23), p.4737-4749
Main Authors: Sun, Han, Nishizawa, Tomoyasu, Ohta, Hiroyuki, Narisawa, Kazuhiko
Format: Article
Language:English
Subjects:
Adaptation
Crops
Ecosystems
Endophytes
Environmental conditions
Flowers & plants
Fungi
Grasses
Growth
Inoculation
Miscanthus
Next-generation sequencing
Nutrients
Organisms
Phylogeny
Plant growth
Plant resistance
Plants
Rice
Sequencing
Toxicity
Vegetation
Volcanic deposits
Volcanoes
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Summary:Growth of the pioneer grass Miscanthus condensatus, one of the first vegetation types to be established on volcanic deposits, is promoted by root-associated fungi, particularly dark septate endophytes (DSEs). Fungal taxa within DSEs colonize the root of Miscanthus condensatus in oligotrophic Andosol, and their function in plant growth promotion remains largely unknown. We, therefore, comprehensively assessed the composition of the DSE community associated with Miscanthus condensatus root in volcanic ecosystems using the approaches of both metabarcoding (next-generation sequencing) and isolation (culturing). Also, the promotion effects of DSEs on plant growth (rice as a proxy) were evaluated by inoculation of core isolates to rice roots. Here, we found the following: (i) 70 % of culturable fungi that colonized Miscanthus condensatus phylogenetically belonged to DSEs; (ii) seven orders were identified by both sequencing and culturing methods; and (iii) inoculation of DSE isolates (Phialocephala fortinii, P. helvetica, and Phialocephala sp.) validated their effects on rice growth, particularly under an extremely low pH condition (compared to the control without inoculation, rice biomass was enhanced 7.6-fold after inoculation of P. fortinii). This study helps improve our understanding of the community of Miscanthus condensatus-associated DSE fungi and their functions in promoting plant growth.
ISSN:1726-4189
1726-4170
1726-4189
DOI:10.5194/bg-20-4737-2023