Delivery of B. subtilis into Animal Intestine Using Chitosan-Derived Bioresorbable Gel Carrier: Preliminary Results

The oral delivery of bacteria in the human intestine is of great interest because of its potential to correct the gut microbiota and treat inflammatory bowel diseases. The aim of this study was to evaluate sodium N-(2-sulfonatoethyl)chitosan gel cross-linked with glutaraldehyde as a delivery carrier...

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Bibliographic Details
Published in:Gels 2023-02, Vol.9 (2), p.120
Main Authors: Samokhin, Alexander, Korel, Anastasia, Blinova, Elena, Pestov, Alexander, Kalmykova, Galina, Akulova, Nadezhda, Betz, Viktoria, Tkachenko, Vadim, Litvinova, Ekaterina
Format: Article
Language:English
Subjects:
bacillus subtilis
Bacteria
Biocompatibility
Biomedical materials
carrier
Chitosan
Communication
Cytotoxicity
Dye releases
Evaluation
Flow cytometry
gel
Homeostasis
In vivo methods and tests
Intestine
Microbiology
Microbiota
Optical microscopy
Probiotics
sodium N-(2-sulfonatoethyl)chitosan
Stomach
Toxicity testing
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Summary:The oral delivery of bacteria in the human intestine is of great interest because of its potential to correct the gut microbiota and treat inflammatory bowel diseases. The aim of this study was to evaluate sodium N-(2-sulfonatoethyl)chitosan gel cross-linked with glutaraldehyde as a delivery carrier for probiotic bacteria to the gut using in vitro and in vivo experiments. The bacterial test strain was . The cytotoxicity of the gel was evaluated via cell culture using flow cytometry and light microscopy. The gel as a delivery system was assessed by the dye release in medium with different pH levels in vitro, and by bacterial titer monitoring in mouse feces using the microbiology method in vivo. Results of an in vitro experiment showed that tested gel has no cytotoxicity. The use of gel as a carrier for bacterial delivery into the intestine was more effective than oral gavage of bacterial suspension. Therefore, gel delivery of bacteria decreased the titer level by up to two times. However, a gavage of bacterial suspension decreased the titer level by over 200 times. Tested gel has the potential to be a carrier for the safe delivery of bacteria to the intestine through the stomach, reducing the rate of the elimination of probiotic bacteria from the intestine.
ISSN:2310-2861
2310-2861
DOI:10.3390/gels9020120