Mir-483 inhibits colon cancer cell proliferation and migration by targeting TRAF1

• Published in: The Kaohsiung journal of medical sciences 2018-09, Vol.34 (9), p.479-486
• Main Authors: Niu, Zi-Yu, Li, Wen-Li, Jiang, Da-Lei, Li, Yan-Song, Xie, Xiang-Jun
• Format: Article
• Language: English
• Subjects: Cell Line, Tumor; Cell Movement - genetics; Cell Movement - physiology; Cell Proliferation - genetics; Cell Proliferation - physiology; Colon cancer; Colon cancer cells; Colonic Neoplasms - genetics; Colonic Neoplasms - metabolism; Gene Expression Regulation, Neoplastic - genetics; Genes; Genetic aspects; Humans; MicroRNA; MicroRNAs - antagonists & inhibitors; MicroRNAs - genetics; MicroRNAs - metabolism; Migration; miR-483; Proliferation; Real-Time Polymerase Chain Reaction; TNF Receptor-Associated Factor 1 - genetics; TNF Receptor-Associated Factor 1 - metabolism; TRAF1
• ISSN: 1607-551X; 2410-8650
• DOI: 10.1016/j.kjms.2018.04.005

MicroRNAs are important regulators during human growth and development. Emerging evidence indicates that microRNAs play important roles in colorectal cancer. The aim of this study is to reveal the biological function and direct target gene of miR-483 in colorectal cancer. The biological function of miR-483 on the proliferation and migration of colon cancer cells was then examined by Edu assay and transwell assay, respectively. Our findings revealed that miR-483 mimic could significantly inhibit cell proliferation and migration. The target gene of miR-483 was predicted by target scan software and identified by a dual fluorescence reporter system which showed that TRAF1 was a direct target gene of miR-483 in SW480 cell line. These data suggest that miR-483 is a colorectal cancer suppressor which could inhibit cell proliferation and migration, possibly via targeting TRAF1. The miR-483 could be a potential treatment target for colorectal cancer.