A Microfluidic Chip Embracing a Nanofiber Scaffold for 3D Cell Culture and Real-Time Monitoring

Recently, three-dimensional (3D) cell culture and tissue-on-a-chip application have attracted attention because of increasing demand from the industries and their potential to replace conventional two-dimensional culture and animal tests. As a result, numerous studies on 3D in-vitro cell culture and...

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Bibliographic Details
Published in:Nanomaterials (Basel, Switzerland) Switzerland), 2019-04, Vol.9 (4), p.588
Main Authors: Kim, Jeong Hwa, Park, Ju Young, Jin, Songwan, Yoon, Sik, Kwak, Jong-Young, Jeong, Young Hun
Format: Article
Language:English
Subjects:
electrospinning
hepatocellular carcinoma cells
live assay
microfluidic chip
nanofibers
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Summary:Recently, three-dimensional (3D) cell culture and tissue-on-a-chip application have attracted attention because of increasing demand from the industries and their potential to replace conventional two-dimensional culture and animal tests. As a result, numerous studies on 3D in-vitro cell culture and microfluidic chip have been conducted. In this study, a microfluidic chip embracing a nanofiber scaffold is presented. A electrospun nanofiber scaffold can provide 3D cell culture conditions to a microfluidic chip environment, and its perfusion method in the chip can allow real-time monitoring of cell status based on the conditioned culture medium. To justify the applicability of the developed chip to 3D cell culture and real-time monitoring, HepG2 cells were cultured in the chip for 14 days. Results demonstrated that the cells were successfully cultured with 3D culture-specific-morphology in the chip, and their albumin and alpha-fetoprotein production was monitored in real-time for 14 days.
ISSN:2079-4991
2079-4991
DOI:10.3390/nano9040588